BONE MARROW STEM CELLS IN FACIAL NERVE REGENERATION FROM ISOLATED STUMPS

Autor(es)

Salomone, Raquel ^[1] ; Bento, Ricardo F. ^[1] ; Costa, Heloisa J. Z. R. ^[1] ; Azzi-Nogueira, Deborah ^[2] ; Ovando, Patricia C. ^[3] ; Da-Silva, Cira F. ^[4] ; Zanatta, Daniela B. ^[5] ; Strauss, Bryan E. ^[5] ; Haddad, Luciana A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Otorhinolaryngol, Sch Med, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Biosci Inst, BR-05403000 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05403000 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Viral Vector Lab, Mol Cardiol Lab LIM 13, Heart Inst, Med Sch, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MUSCLE & NERVE; v. 48, n. 3, p. 423-429, SEP 2013.
• Citações Web of Science: 17

Resumo

IntroductionSevere lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. Methods: We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann-like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated. Results: Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B. Conclusions: Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results. (AU)

• Processo FAPESP: 08/53857-8 - Splicing alternativo e diversidade funcional do gene FMR1
• Beneficiário: Luciana Amaral Haddad
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/14254-2 - Centro de Estudos do Genoma Humano
• Beneficiário: Mayana Zatz
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 08/00458-9 - Avaliação da regeneração do nervo facial de ratos após a implantação de células tronco derivadas do estroma de medula óssea diferenciadas in vitro
• Beneficiário: Ricardo Ferreira Bento
• Modalidade de apoio: Auxílio à Pesquisa - Regular