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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

BONE MARROW STEM CELLS IN FACIAL NERVE REGENERATION FROM ISOLATED STUMPS

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Author(s):
Salomone, Raquel [1] ; Bento, Ricardo F. [1] ; Costa, Heloisa J. Z. R. [1] ; Azzi-Nogueira, Deborah [2] ; Ovando, Patricia C. [3] ; Da-Silva, Cira F. [4] ; Zanatta, Daniela B. [5] ; Strauss, Bryan E. [5] ; Haddad, Luciana A. [2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Otorhinolaryngol, Sch Med, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Biosci Inst, BR-05403000 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, BR-05403000 Sao Paulo - Brazil
[5] Univ Sao Paulo, Viral Vector Lab, Mol Cardiol Lab LIM 13, Heart Inst, Med Sch, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: MUSCLE & NERVE; v. 48, n. 3, p. 423-429, SEP 2013.
Web of Science Citations: 17
Abstract

IntroductionSevere lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. Methods: We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann-like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated. Results: Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B. Conclusions: Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results. (AU)

FAPESP's process: 98/14254-2 - The Human Genome Research Center
Grantee:Mayana Zatz
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 08/00458-9 - Evaluation of the facial nerve regeneration in rat after vitro differentiation bone marrow stromal cells implantation
Grantee:Ricardo Ferreira Bento
Support Opportunities: Regular Research Grants