Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets

Autor(es)

da Silva Nassar, Ana Ligia ^[1] ; Marot, Luisa Pereira ^[1] ; Ovidio, Paula Payao ^[2] ; Ferreira de Castro, Gabriela Salim ^[3] ; Jordao Junior, Alceu Afonso ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, BR-14049 Ribeirao Preto, SP - Brazil

^[2] FMRP USP, Dept Internal Med, Lab Nutr & Metab, Ribeirao Preto, SP - Brazil

^[3] FMRP USP, Dept Internal Med, Postgrad Program Internal Med, Ribeirao Preto, SP - Brazil

^[4] FMRP USP, Dept Internal Med, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 29, n. 3, p. 178-185, MAR 2014.
• Citações Web of Science: 3

Resumo

PURPOSE: To assess oxidative stress and the profile of fatty acids incorporated into the hepatic tissue of animals refed with high-fat (HF) diets after acute food restriction. METHODS: Fifty male Wistar rats were divided into five groups and fasting for 48 hours. One group was sacrificed without refeeding (NR), a control group (C) was refed with the standard AIN-93 diet and the remaining groups with HF diets respectively consisting of hydrogenated vegetable oil (PHVO), trans-free (TF) margarine and trans-free margarine enriched with omega-3 and omega-6 (O). After this period the animals were sacrificed for malondialdehyde (MDA), catalase and hepatic fatty acid determination. RESULTS: The groups refed with HF diets showed elevation of MDA levels compared to the C group (p<0.001 for GVH and p<0.01 for TF and O). Hepatic catalase activity was higher in the TF and O groups compared to group C (p<0.05 for both). The amount of saturated fatty acids was lower in the PHVO and O groups compared to the remaining ones (p<0.001). CONCLUSION: The consumption of high-fat diets after prolonged fasting favors oxidative imbalance in hepatic tissue. (AU)

• Processo FAPESP: 10/00408-1 - Efeitos dos Ácidos Graxos N-3 Provenientes do Óleo de Peixe no Metabolismo Lipídico e Expressão Gênica de Ratos Alimentados com Dieta Rica em Frutose
• Beneficiário: Gabriela Salim Ferreira de Castro
• Modalidade de apoio: Bolsas no Brasil - Doutorado