PROTECTIVE EFFECT OF ESTRADIOL ON ACUTE LUNG INFLAMMATION INDUCED BY AN INTESTINAL ISCHEMIC INSULT IS DEPENDENT ON NITRIC OXIDE

Autor(es)

Breithaupt-Faloppa, A. C. ^[1] ; Fantozzi, E. T. ^[2] ; Assis-Ramos, M. M. ^[2] ; Vitoretti, L. B. ^[2] ; Couto, G. K. ^[3] ; Rossoni, L. V. ^[4] ; Oliveira-Filho, R. M. ^[3] ; Vargaftig, B. B. ^[2] ; Tavares-de-Lima, W. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Cardiovasc Surg & Physiopathol Circulat LIM 1, Heart Inst InCor, Sch Med, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Shock; v. 40, n. 3, p. 203-209, SEP 2013.
• Citações Web of Science: 22

Resumo

Introduction: It has been shown that the innate immune system mediates acute lung inflammation triggered by intestinal trauma. Sexual dimorphism modulates the profile of T(H)1 and T(H)2 lymphocytes, and accordingly sex hormones may modulate acute lung inflammation by intestinal ischemia/reperfusion (I/R). Studies indicate that female rats are relatively resistant to organ injury caused by hemorrhagic shock and that the gut of female is more resistant than that of the male to deleterious effects of ischemic injury. At the present study, we investigated the effect of estradiol (E-2) on the lung inflammation after intestinal I/R and its interaction with the nitric oxide (NO) pathway. Methods: Anesthetized female rats submitted or not to 7 days ovariectomy (OVx) were subjected to occlusion of the superior mesenteric artery during 45 min, followed by 2 h of reperfusion. Groups of rats were treated with E2 (17 beta-estradiol, 280 mu g/kg, s.c.) 24 h before ischemia and/or with the nonselective NO synthase inhibitor L-NAME (N5-nitro-L-arginine methyl ester hydrochloride) (5 mg/kg, i.v.). In a parallel set of experiments, the selective NO synthase inhibitor, aminoguanidine (50 mg/kg i.v.), was given 1 h before ischemia. In all groups, lung vascular permeability (LVP) was assessed using the Evans blue dye extravasation method, neutrophil recruitment to the tissues by the standard myeloperoxidase (MPO) method, and endothelial NO synthase (eNOS) protein expression by Western blot. Results: In OVx rats, LVP and MPO were increased after intestinal I/R as compared with intact controls. Estradiol reverted the LVP, but not MPO. Aminoguanidine reduced LVP in OVx rats. The E2 protective effect on LVP was abolished by L-NAME; moreover, an increase in LVP even when compared with OVx rats treated only with L-NAME was observed. In addition, lung eNOS protein expression was reduced in OVx-I/R rats in comparison to intact controls and the E2 inhibited this effect. Conclusions: Estradiol treatment is able to reduce lung inflammation due to intestinal I/R, but with the concomitant blockade of NOS activity, this effect is abolished. Nitric oxide probably reduces the vascular deleterious effects of intestinal I/R, and E2 pretreatment reduces lung inflammation after intestinal I/R and exerts these effects by modulating eNOS protein expression in the lungs. (AU)

• Processo FAPESP: 09/54823-2 - Avaliacao do papel dos hormonios sexuais femininos e do sistema linfatico no controle da inflamacao pulmonar e sistemica apos a isquemica e reperfusao intestinal em ratas
• Beneficiário: Wothan Tavares de Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular