Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D-3 supplementation

Autor(es)

Rezende, L. R. ^[1] ; Delgado, E. F. ^[1] ; Junior, A. R. L. ^[2] ; Gasparin, G. ^[1] ; Jorge, E. C. ^[3] ; Mourao, G. B. ^[1] ; Coutinho, L. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Zootecnia, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Dept Ciencias Basicas, Fac Zootecnia & Engn Alimentos, Pirassununga, SP - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morfol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 12, n. 4, p. 6611-6618, 2013.
• Citações Web of Science: 0

Resumo

In order to better understand vitamin D-3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 10(6) international units of vitamin D-3 administered for 2 or 8 consecutive days pre-slaughter). Vitamin D-3 supplementation resulted in a significant increase in 1alpha-HYD gene expression; however, significantly increased 24-HYD gene expression was only detected in cattle that had 8 days of supplementation. The finding of upregulation of 24-HYD due to vitamin D supplementation is in line with the expected rise in 24,25-di-hydroxy-vitamin D-3 synthesis observed when plasma vitamin D-3 concentrations are high, stimulating excretion by the organism. On the other hand, upregulation of 1alpha-HYD was unexpected, since vitamin D-3 supplementation has been reported to impact these two genes in opposite directions. We conclude that vitamin D-3 metabolism in these animals is more complex than previously reported. (AU)

• Processo FAPESP: 06/06963-1 - Interação da suplementação de vitamina D3 na dieta com a exposição solar nas características qualitativas da carne bovina
• Beneficiário: Eduardo Francisquine Delgado
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/01986-9 - Expressão de genes relacionados ao metabolismo de vitamina d3 mediante suplementação e estudo de associação com a maciez da carne em bovinos da raça nelore
• Beneficiário: Lilian Ribeiro Rezende
• Modalidade de apoio: Bolsas no Brasil - Mestrado