Influence of TLR-2 in the immune response in the infection induced by fungus Sporothrix schenckii

Autor(es)

Negrini, Thais de C. ^[1] ; Ferreira, Lucas S. ^[1] ; Arthur, Rodrigo A. ^[2] ; Alegranci, Pamela ^[1] ; Placeres, Marisa C. P. ^[1] ; Spolidorio, Luis C. ^[3] ; Carlos, Iracilda Z. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 Araraquara, SP - Brazil

^[2] Univ Fed Rio Grande do Sul, Sch Dent, Dept Prevent & Community Dent, BR-90046900 Porto Alegre, RS - Brazil

^[3] Sao Paulo State Univ, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801902 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IMMUNOLOGICAL INVESTIGATIONS; v. 43, n. 4, p. 370-390, 2014.
• Citações Web of Science: 12

Resumo

Toll-like receptors (TLRs) play an important role in immunity, since they bind to pathogen surface antigens and initiate the immune response. However, little is known about the role of TLR-2 in the recognition of S. schenckii and in the subsequent immune response. Therefore, the aim of this study was to evaluate the involvement of TLR-2 in the immune response induced by S. schenckii. C57BL/6 mice (WT) and C57BL/6 TLR-2 knockout (TLR-2(-/-)) were used to evaluate, over a period of 10 weeks of sporotrichotic infection, the influence of TLR-2 over macrophages production of IL-1 beta, IL-12 and TNF-alpha, their stimulation level by NO release and the production of IFN-gamma, IL-6, IL-17 and TGF-beta by spleen cells. The results showed that the production of pro-inflammatory mediators and NO, TLR-2 interference is striking, since its absence completely inhibited it. IL-17 production was independent of TLR-2. The absence of Th1 response in TLR2(-/-) animals was concomitant with IL-17 production. Therefore, it can be suggested that TLR-2 absence interferes with the course of the infection induced by the fungus S. schenckii. (AU)

• Processo FAPESP: 09/11999-3 - Influência dos receptores TLR-2 e TLR-4 na defesa do hospedeiro contra o fungo Sporothrix schenckii
• Beneficiário: Iracilda Zeppone Carlos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/07529-1 - Influência do receptor TLR-2 na defesa do hospedeiro contra o fungo Sporothrix schenckii
• Beneficiário: Thais de Cassia Negrini
• Modalidade de apoio: Bolsas no Brasil - Doutorado