Adenovirus Vector-Induced CD8(+) T Effector Memory Cell Differentiation and Recirculation, But Not Proliferation, Are Important for Protective Immunity Against Experimental Trypanosoma cruzi Infection

Autor(es):Mostrar menos -

Vasconcelos, Jose Ronnie ^{[1, 2]} ; Dominguez, Mariana R. ^{[1, 3]} ; Neves, Ramon L. ^{[1, 3]} ; Ersching, Jonatan ^{[1, 3]} ; Araujo, Adriano ^{[1, 3]} ; Santos, Luara I. ^[4] ; Virgilio, Fernando S. ^{[1, 3]} ; Machado, Alexandre V. ^[5] ; Bruna-Romero, Oscar ^[6] ; Gazzinelli, Ricardo T. ^{[5, 4, 7]} ; Rodrigues, Mauricio M. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, CTCMol, Escola Paulista Med, BR-04044010 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, BR-11015020 Santos, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, BR-04044010 Sao Paulo - Brazil

^[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG - Brazil

^[5] Fiocruz MS, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG - Brazil

^[6] Univ Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, BR-88040900 Florianopolis, SC - Brazil

^[7] Univ Massachusetts, Div Infect Dis & Immunol, Dept Med, Sch Med, Worcester, MA 01655 - USA

• Tipo de documento: Artigo Científico
• Fonte: Human Gene Therapy; v. 25, n. 4, p. 350-363, APR 1 2014.
• Citações Web of Science: 10

Resumo

Heterologous prime-boost vaccination using plasmid DNA followed by replication-defective adenovirus vector generates a large number of specific CD8(+) T effector memory (TEM) cells that provide long-term immunity against a variety of pathogens. In the present study, we initially characterized the frequency, phenotype, and function of these T cells in vaccinated mice that were subjected to infectious challenge with the human protozoan parasite Trypanosoma cruzi. We observed that the frequency of the specific CD8(+) T cells in the spleens of the vaccinated mice increased after challenge. Specific TEM cells differentiated into cells with a KLRG1(High) CD27(Low) CD43(Low) CD183(Low)T-bet(High)Eomes(Low) phenotype and capable to produce simultaneously the antiparasitic mediators IFN and TNF. Using the gzmBCreERT2/ROSA26EYFP transgenic mouse line, in which the cells that express Granzyme B after immunization, are indelibly labeled with enhanced yellow fluorescent protein, we confirmed that CD8(+) T cells present after challenge were indeed TEM cells that had been induced by vaccination. Subsequently, we observed that the in vivo increase in the frequency of the specific CD8(+) T cells was not because of an anamnestic immune response. Most importantly, after challenge, the increase in the frequency of specific cells and the protective immunity they mediate were insensitive to treatment with the cytostatic toxic agent hydroxyurea. We have previously described that the administration of the drug FTY720, which reduces lymphocyte recirculation, severely impairs protective immunity, and our evidence supports the model that when large amounts of antigen-experienced CD8(+) TEM cells are present after heterologous prime-boost vaccination, differentiation, and recirculation, rather than proliferation, are key for the resultant protective immunity. (AU)

• Processo FAPESP: 09/06820-4 - Caracterização das células apresentadoras de antígeno capazes de iniciar a reposta imune e controlar a imunodominância de Linfócitos T CD8+ específicos durante a infecção experimental pelo Trypanosoma cruzi
• Beneficiário: Maurício Martins Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/22514-3 - Estudo da migração de células T específicas geradas pela vacinação ou infecção pelo Trypanosoma cruzi
• Beneficiário: Jose Ronnie Carvalho de Vasconcelos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores