ApoptomiRs expression modulated by BCR-ABL is linked to CML progression and imatinib resistance

Autor(es):Mostrar menos -

Ferreira, A. F. ^[1] ; Moura, L. G. ^[1] ; Tojal, I. ^[2] ; Ambrosio, L. ^[1] ; Pinto-Simoes, B. ^[3] ; Hamerschlak, N. ^[4] ; Calin, G. A. ^[5] ; Ivan, C. ^[6] ; Covas, D. T. ^{[2, 3]} ; Kashima, S. ^[2] ; Castro, F. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeiro Preto, Dept Anal Clin Toxicol & Bromatolog, BR-05508 Sao Paulo - Brazil

^[2] Ctr Reg Hemoterapia Ribeirao Preto, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, BR-05508 Sao Paulo - Brazil

^[4] Hosp Israelita Albert Einstein, Sao Paulo - Brazil

^[5] Univ Texas MD Anderson Canc Ctr, Dept Expt Therapeut, Houston, TX 77030 - USA

^[6] Univ Texas MD Anderson Canc Ctr, Ctr RNA Interference & Noncoding RNAs, Houston, TX 77030 - USA

• Tipo de documento: Artigo Científico
• Fonte: BLOOD CELLS MOLECULES AND DISEASES; v. 53, n. 1-2, p. 47-55, JUN-AUG 2014.
• Citações Web of Science: 14

Resumo

Background: Chronic myeloid leukemia (CML) is a myeloproliferative disease characterized by the presence of Philadelphia chromosome (Ph) leading to expression of a BCR-ABL1 fusion oncogene. The BCR-ABL protein has a constitutive tyrosine kinase activity which is responsible for CML pathogenesis by promoting cell apoptosis resistance; however, the cellular and molecular mechanisms associated with BCR-ABL expression and apoptosis impairment in CML leukemic cells have not been fully elucidated. Methods: This study evaluated apoptomiRs and their predicted apoptotic genes in BCR-ABL(+) cells from patients in different phases of CML treated with tyrosine kinase inhibitor (TKI) according to their imatinib (IM) response by qPCR Phosphotyrosine and c-ABL expressions in HL-60.BCR-ABL cells treated with TKI were done by Western blot. Results: We found that dasatinib (DAS) modulated miR-let-7d, miR-let-7e, miR-15a, miR-16, miR-21, miR-130a and miR-142-3p expressions while IM modulated miR-15a and miR-130a levels. miR-16, miR-130a and miR-145 expressions were modulated by nilotinib (NIL). We observed higher miR-15a, miR-130b and miR-145; and lower miR-16, miR-26a and miR-146a expressions in CML-CP in comparison with controls. CML-AP patients showed low miR-let-7d, miR-15a, miR-16, miR-29c, miR-142-3p, miR-145, and miR-146a levels in comparison with CML-CP. We noted that the miR-26a, miR-29c, miR-130b and miR-146a expressions were downregulated in IM resistant patients in comparison with IM responsive patients. Conclusions: This study showed the modulation of apoptomiRs by BCR-ABL kinase activity and the deregulation of apoptomiRs and their predicted apoptotic target genes in different CML phases and after treatment with TK inhibitors. ApoptomiRs may be involved in the BCR-ABL+ cell apoptosis regulation. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/57746-8 - Efeito dos inibidores de tirosina-quinase na maquinaria apoptotica na leucemia mieloide cronica.
• Beneficiário: Aline Fernanda Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/20135-2 - Análise epigenética e molecular de neoplasias mieloproliferativas crônicas JAK2 v617f positivas e negativas
• Beneficiário: Fabíola Attié de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Regular