Abundance of MMPs and Cysteine Cathepsins in Caries-affected Dentin

Autor(es)

Vidal, C. M. P. ^[1] ; Tjaderhane, L. ^{[2, 3]} ; Scaffa, P. M. ^[1] ; Tersariol, I. L. ^{[4, 5]} ; Pashley, D. ^[6] ; Nader, H. B. ^[4] ; Nascimento, F. D. ^{[7, 8]} ; Carrilho, M. R. ^{[9, 7, 8]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dent Mat Area, Dept Restorat Dent, Piracicaba - Brazil

^[2] Univ Oulu, Inst Dent, Oulu - Finland

^[3] Oulu Univ Hosp, Oulu - Finland

^[4] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo - Brazil

^[5] Univ Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, Mogi das Cruzes - Brazil

^[6] Georgia Regents Univ, Dept Oral Biol, Coll Dent Med, Augusta, GA - USA

^[7] Univ Bandeirante Sao Paulo, Biomat Res Grp, Sao Paulo - Brazil

^[8] Univ Bandeirante Sao Paulo, Div Biotechnol, Sao Paulo - Brazil

^[9] Univ Western Ontario, Schulich Sch Med & Dent, London, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DENTAL RESEARCH; v. 93, n. 3, p. 269-274, MAR 2014.
• Citações Web of Science: 56

Resumo

Degradation of dentin matrix components within caries dentin has been correlated with the activity of host-derived proteases, such as matrix metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship has not been fully established, we hypothesized that the abundance of MMPs and CTs in caries-affected dentin must be higher than in intact dentin. To test this premise, we obtained 5 slices (200 mu m) from 5 intact teeth and from 5 caries-affected teeth (1 slice/tooth) and individually incubated them with primary antibodies for CT-B, CT-K, MMP-2, or MMP-9. Negative controls were incubated with pre-immune serum. Specimens were washed and re-incubated with the respective fluorescent secondary antibody. Collagen identification, attained by the autofluorescence capture technique, and protease localization were evaluated by multi-photon confocal microscopy. The images were analyzed with ZEN software, which also quantitatively measured the percentages of collagen and protease distribution in dentin compartments. The abundance of the test enzymes was markedly higher in caries-affected than in intact dentin. CT-B exhibited the highest percentage of co-localization with collagen, followed by MMP-9, MMP-2, and CT-K. The high expression of CTs and MMPs in caries-affected teeth indicates that those host-derived enzymes are intensely involved with caries progression. (AU)

• Processo FAPESP: 13/05822-9 - Receptores ativados por proteases (PARs) no complexo dentina-polpa: identificação, modulação e sinalização celular durante o desenvolvimento da doença cárie
• Beneficiário: Fábio Dupart Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/12226-8 - Avaliação da atividade de proteases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 09/13652-0 - Estudo da Atividade Proteolítica da Dentina Humana Sadia e Afetada por Cárie.
• Beneficiário: Cristina de Mattos Pimenta Vidal
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/54618-4 - Avaliação da atividade de colagenases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores