Influence of Adhesive Restorations on Diffusion of H2O2 Released from a Bleaching Agent and Its Toxic Effects on Pulp Cells

Autor(es)

Soares, Diana Gabriela ^[1] ; Pastana, Julia Vieira ^[2] ; de Oliveira Duque, Carla Caroline ^[2] ; Dias Ribeiro, Ana Paula ^[3] ; Basso, Fernanda Goncalves ^[4] ; Hebling, Josimeri ^[5] ; de Souza Costa, Carlos Alberto ^[2]

Afiliação do(s) autor(es)

^[1] UNESP, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

^[3] Fed Univ Brasilia, Dept Dent, Brasilia, DF - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP - Brazil

^[5] UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Adhesive Dentistry; v. 16, n. 2, p. 123-128, 2014.
• Citações Web of Science: 5

Resumo

Purpose: To assess the influence of adhesive restorations on hydrogen peroxide (H2O2) diffusion through enamel and dentin and its cytotoxicity to pulp (MDPC-23) cells. Materials and Methods: Sound and resin-restored enamel/dentin disks were stored in water for 24h or 6 months and adapted to artificial pulp chambers. Bleaching gels with 20% or 35% H2O2 were applied to the enamel surface for 45 min, and a culture medium in direct contact with the dentin surface (extract) was applied for 1 h to the MDPC-23 cells. Cell metabolism (MU assay) and cell morphology (SEM) were assessed. The amount of H2O2 in the extracts was also quantified (peroxidase/leuco-crystal violet reaction). Results: A significant reduction in cell metabolism was observed between the group bleached with the 35% gel and the control group (sound, nonbleached) (p < 0.05). The H2O2 diffusion was directly related to its concentration in the bleaching gel. The variables ``presence of restoration{''} and ``time of water storage{''} did not significantly influence H2O2 diffusion or cell metabolism for either of the bleaching gels (p > 0.05). All bleached groups presented alterations in cell morphology related to the concentration of H2O2 in the bleaching gel. Conclusion: The reduction in cell metabolism and the changes in cell morphology were H2O2-concentration dependent, having no relationship with the presence of either new or aged adhesive restorations on teeth subjected to bleaching therapies. (AU)

• Processo FAPESP: 11/12938-8 - Novos parâmetros para o clareamento dental: avaliação da eficácia, citotoxicidade e efeitos moleculares
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/09385-7 - Novos parâmetros para o clareamento dental: avaliação da eficácia, citotoxicidade e efeitos moleculares
• Beneficiário: Diana Gabriela Soares dos Passos
• Modalidade de apoio: Bolsas no Brasil - Doutorado