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Molecular and Kinetic Characterization of Two Extracellular Xylanases Isolated from Leucoagaricus gongylophorus

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Autor(es):
Moreira, Ariele C. [1] ; Ferreira, Douglas [1] ; de Almeida, Fernando G. [1] ; Rodrigues-Filho, Edson [1] ; Fernandes, Joao B. [1] ; das Gracas Fernandes da Silva, Maria Fatima [1] ; Vieira, Paulo C. [1] ; Pagnocca, Fernando C. [2] ; Souza, Dulce Helena F. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Quim, Sao Paulo - Brazil
[2] UNESP Sao Paulo State Univ, Ctr Study Social Insects, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Applied Biochemistry and Biotechnology; v. 173, n. 3, p. 694-704, JUN 2014.
Citações Web of Science: 4
Resumo

In this work, the xylanolytic profile of Leucoagaricus gongylophorus was studied, and two extracellular enzymes with xylanolytic activity (XyLg1 and XyLg2) were isolated, purified, and characterized. XyLg1 has a molecular mass of about 38 kDa and pI greater than 4.8. For beechwood xylan substrate, XyLg1 showed an optimum temperature of 40 A degrees C, optimum pH between 8.5 and 10.5, and Km = 14.7 A +/- 7.6 mg mL(-1). Kinetic studies of the XyLg1 using polygalacturonic acid as substrate were developed, and the enzyme showed optimum pH 5.5, optimum temperature between 50 and 60 A degrees C, and Km = 2.2 A +/- 0.5 mg mL(-1). XyLg2 has molecular weight of about 24 kDa and pI less than 4.8, and thus is an acid protein. Parameters such as optimum temperature (70 A degrees C) and pH (4.0), as well as the kinetic parameters (Km = 7.4 A +/- 2.0 mg mL(-1)) using beechwood xylan as substrate, were determined for XyLg2. This enzyme has no activity for polygalacturonic acid as substrate. XyLg1 and XyLg2 are the first native xylanases isolated and characterized from L. gongylophorus fungi and, due to their biochemistry and kinetic features, they have potential to be used in biotechnological processes. (AU)

Processo FAPESP: 11/21955-3 - Enzimas lignocelulolíticas produzidas por fungos: estudos funcional e estrutural
Beneficiário:Dulce Helena Ferreira de Souza
Modalidade de apoio: Auxílio à Pesquisa - Regular