NMDA and AMPA/Kainate Glutamatergic Receptors in the Prelimbic Medial Prefrontal Cortex Modulate the Elaborated Defensive Behavior and Innate Fear-Induced Antinociception Elicited by GABA(A) Receptor Blockade in the Medial Hypothalamus

Autor(es)

de Freitas, Renato Leonardo ^{[1, 2]} ; Salgado-Rohner, Carlos Jose ^[1] ; Biagioni, Audrey Francisco ^{[1, 2]} ; Medeiros, Priscila ^{[1, 3, 2]} ; Cecilio Hallak, Jaime Eduardo ^{[4, 5]} ; Crippa, Jose Alexandre S. ^{[4, 5]} ; Coimbra, Norberto Cysne ^{[1, 2, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Farmacol, Lab Neuroanat & Neuropsicobiol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Inst Neurociencias & Comportamento INeC, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, BR-1060001 Santos, SP - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Neurociencias & Ciencias Comportament, Setor Psiquiatria, BR-14049900 Ribeirao Preto, SP - Brazil

^[5] Univ Fed Rio Grande do Sul, CNPq, Natl Inst Sci & Translat Technol Med INCT TM, Porto Alegre, RS - Brazil

^[6] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Farmacol, NAP USP Neurobiol Emocoes NuPNE, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CEREBRAL CORTEX; v. 24, n. 6, p. 1518-1528, JUN 2014.
• Citações Web of Science: 15

Resumo

The aim of the present study was to investigate the involvement of N-methyl-d-aspartate (NMDA) and amino-3-hydroxy-5-methyl-isoxazole-4-proprionate (AMPA)/kainate receptors of the prelimbic (PL) division of the medial prefrontal cortex (MPFC) on the panic attack-like reactions evoked by gamma-aminobutyric acid-A receptor blockade in the medial hypothalamus (MH). Rats were pretreated with NaCl 0.9%, LY235959 (NMDA receptor antagonist), and NBQX (AMPA/kainate receptor antagonist) in the PL at 3 different concentrations. Ten minutes later, the MH was treated with bicuculline, and the defensive responses were recorded for 10 min. The antagonism of NMDA receptors in the PL decreased the frequency and duration of all defensive behaviors evoked by the stimulation of the MH and reduced the innate fear-induced antinociception. However, the pretreatment of the PL cortex with NBQX was able to decrease only part of defensive responses and innate fear-induced antinociception. The present findings suggest that the NMDA-glutamatergic system of the PL is critically involved in panic-like responses and innate fear-induced antinociception and those AMPA/kainate receptors are also recruited during the elaboration of fear-induced antinociception and in panic attack-related response. The activation of the glutamatergic neurotransmission of PL division of the MPFC during the elaboration of oriented behavioral reactions elicited by the chemical stimulation of the MH recruits mainly NMDA receptors in comparison with AMPA/kainate receptors. (AU)

• Processo FAPESP: 03/05256-1 - Caracterizacao de receptores serotoninergicos na analgesia pos-ictal: envolvimento do nucleo dorsal da rafe.
• Beneficiário: Renato Leonardo de Freitas
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/08955-1 - Envolvimento das redes neurais do núcleo dorsal da rafe e da neurotransmissão serotoninérgica no locus coeruleus em processos antinociceptivos induzidos por reações de fuga orientada evocada pela estimulação química do hipotálamo medial
• Beneficiário: Audrey Franceschi Biagioni
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 01/03752-6 - Estudo do envolvimento de receptores m-opioides, colinérgicos muscarínicos e nicotínicos e serotonérgicos 5-HT2/1C na analgesia pós-ictal
• Beneficiário: Renato Leonardo de Freitas
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/15140-4 - Estudo neurofarmacológico do envolvimento do sistema serotoninérgico do núcleo dorsal da rafe na elaboração da antinocicepção induzida por reações de fuga elaborada causada pela desinibição GABAérgica do hipotálamo dorsomedial
• Beneficiário: Audrey Franceschi Biagioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/14446-2 - Participação do substrato neural glutamatérgico do colículo inferior sobre a catalepsia induzida pela microinjeção intraestriatal de Haloperidol
• Beneficiário: Priscila Medeiros de Freitas
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/17258-5 - Estudo do envolvimento do sistema nitrérgico e da neurotransmissão canabinoide e glutamatérgica do córtex pré-frontal medial na analgesia induzida por reações de fuga elaborada evocada pelo bloqueio GABAérgico do hipotálamo medial
• Beneficiário: Renato Leonardo de Freitas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado