Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification

Autor(es)

Santos, Rodrigo I. M. ^[1] ; Rodrigues, Alcir H. ^[2] ; Silva, Maria Lucia ^[3] ; Mortara, Renato A. ^[4] ; Rossi, Marcos A. ^[5] ; Jamur, Maria Celia ^[6] ; Oliver, Constance ^[7] ; Arruda, Eurico ^[8]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo. Sch Med Ribeirao Preto

^[2] Univ Sao Paulo. Sch Med Ribeirao Preto

^[3] Univ Sao Paulo. Sch Med Ribeirao Preto

^[4] Univ Fed Sao Paulo. Escola Paulista Med

^[5] Univ Sao Paulo. Sch Med Ribeirao Preto

^[6] Univ Sao Paulo. Sch Med Ribeirao Preto

^[7] Univ Sao Paulo. Sch Med Ribeirao Preto

^[8] Univ Sao Paulo. Sch Med Ribeirao Preto

• Tipo de documento: Artigo Científico
• Fonte: VIRUS RESEARCH; v. 138, n. 1-2, p. 139-143, DEC 2008.
• Citações Web of Science: 14

Resumo

Oropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. The data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associates with clathrin-coated pits and can be found in association with late endosomes in a time shorter than 1 h. (C) 2008 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 03/03682-3 - Estudos sobre arboviroses e viroses associadas a roedores
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Modalidade de apoio: Auxílio à Pesquisa - Temático