Diet-induced obesity induces endoplasmic reticulum stress and insulin resistance in the amygdala of rats

Autor(es)

Castro, Gisele ^[1] ; Areias, Maria Fernanda C. ^[2] ; Weissmann, Lais ^[2] ; Quaresma, Paula G. F. ^[2] ; Katashima, Carlos K. ^[2] ; Saad, Mario J. A. ^[2] ; Prada, Patricia O. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Appl Sci, Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Dept Internal Med, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS OPEN BIO; v. 3, p. 443-449, 2013.
• Citações Web of Science: 36

Resumo

Insulin acts in the hypothalamus, decreasing food intake (FI) by the IR/PI3K/Akt pathway. This pathway is impaired in obese animals and endoplasmic reticulum (ER) stress and low-grade inflammation are possible mechanisms involved in this impairment. Here, we highlighted the amygdala as an important brain region for FI regulation in response to insulin. This regulation was dependent on PI3K/AKT pathway similar to the hypothalamus. Insulin was able to decrease neuropeptide Y (NPY) and increase oxytocin mRNA levels in the amygdala via PI3K, which may contribute to hypophagia. Additionally, obese rats did not reduce FI in response to insulin and AKT phosphorylation was decreased in the amygdala, suggesting insulin resistance. Insulin resistance was associated with ER stress and low-grade inflammation in this brain region. The inhibition of ER stress with PBA reverses insulin action/signaling, decreases NPY and increases oxytocin mRNA levels in the amygdala from obese rats, suggesting that ER stress is probably one of the mechanisms that induce insulin resistance in the amygdala. (C) 2013 The Authors. Published by Elsevier B.V. on behalf of Federation of European Biochemical Societies. All rights reserved. (AU)

• Processo FAPESP: 08/55674-8 - Regulação da proteína TUB em núcleos hipotalâmicos: efeitos da leptina, insulina, jejum, idade e dieta hiperlipídica
• Beneficiário: Patrícia de Oliveira Prada
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores