Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli

Autor(es)

Cassado, Alexandra dos Anjos ^{[1, 2, 3]} ; Tavares de Albuquerque, Jose Antonio ^[2] ; Sardinha, Luiz Roberto ^[4] ; Buzzo, Carina de Lima ^{[1, 2, 3]} ; Faustino, Lucas ^[2] ; Nascimento, Rogerio ^[2] ; Ghosn, Eliver Eid Bou ^[5] ; D'Imperio Lima, Maria Regina ^[2] ; Mosig Alvarez, Jose Maria ^[2] ; Bortoluci, Karina Ramalho ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Ciencias Biol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Ctr Terapia Celular & Mol CTC Mol, Sao Paulo - Brazil

^[4] Inst Israelita Ensino & Pesquisa Albert Einstein, Sao Paulo - Brazil

^[5] Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 - USA

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 6, n. 7 JUL 22 2011.
• Citações Web of Science: 18

Resumo

The peritoneal cavity (PerC) is a singular compartment where many cell populations reside and interact. Despite the widely adopted experimental approach of intraperitoneal (i.p.) inoculation, little is known about the behavior of the different cell populations within the PerC. To evaluate the dynamics of peritoneal macrophage (Mempty set) subsets, namely small peritoneal Mempty set (SPM) and large peritoneal Mempty set (LPM), in response to infectious stimuli, C57BL/6 mice were injected i.p. with zymosan or Trypanosoma cruzi. These conditions resulted in the marked modification of the PerC myelo-monocytic compartment characterized by the disappearance of LPM and the accumulation of SPM and monocytes. In parallel, adherent cells isolated from stimulated PerC displayed reduced staining for beta-galactosidase, a biomarker for senescence. Further, the adherent cells showed increased nitric oxide (NO) and higher frequency of IL-12-producing cells in response to subsequent LPS and IFN-gamma stimulation. Among myelo-monocytic cells, SPM rather than LPM or monocytes, appear to be the central effectors of the activated PerC; they display higher phagocytic activity and are the main source of IL-12. Thus, our data provide a first demonstration of the consequences of the dynamics between peritoneal Mempty set subpopulations by showing that substitution of LPM by a robust SPM and monocytes in response to infectious stimuli greatly improves PerC effector activity. (AU)

• Processo FAPESP: 08/50958-8 - Interação entre receptores da imunidade inata na ativação de macrófagos e células dendríticas
• Beneficiário: Karina Ramalho Bortoluci
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores