Tyrosine phosphorylation plays a role in increasing maspin protein levels and its cytoplasmic accumulation

Autor(es)

Longhi, Mariana Tamazato ^{[1, 2]} ; Cella, Nathalie ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Biol Celular & Desenvolvimento, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS OPEN BIO; v. 2, p. 93-97, 2012.
• Citações Web of Science: 7

Resumo

Maspin is a tumor suppressor with many biological activities, multiple ligands and different subcellular localizations. Its underlying molecular mechanism remains elusive. We hypothesized that phosphorylation might regulate maspin localization and function. Using two-dimensional gel electrophoresis with different focusing power followed by Western blot we identified four different maspin forms with the same molecular weight (42 kDa), but different isoelectric points. Three of these forms were sensitive to acidic phosphatase treatment, suggesting that they are phosphorylated. Sodium peroxidovanadate treatment, a protein-tyrosine phosphatase inhibitor, resulted in a rapid increase in maspin protein levels and cytoplasmic accumulation. These data show that there are three different maspin tyrosine phosphoforms. Inhibition of tyrosine phosphatases increased maspin protein levels and leads to its cytoplasmic accumulation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. (AU)

• Processo FAPESP: 05/60353-8 - O papel da maspina na adesão, morfogênese e morte celular
• Beneficiário: Nathalie Cella
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/12597-6 - Caracterização de alterações pós-traducionais de maspina e correlação com diferentes localizações subcelulares
• Beneficiário: Mariana Tamazato Longhi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/53590-6 - O papel da maspina na adesao, morfogenese e morte celular.
• Beneficiário: Nathalie Cella
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores