Effects of early weaning on molecular markers during postnatal growth and development of intestinal mucosa in the rat.

• Grant number: 18/07782-8
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2018
• End date: June 30, 2020
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Patrícia Gama
• Grantee: Patrícia Gama
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 20/02888-2 - Effects of early weaning on molecular markers during postnatal growth and development of intestinal, BP.TT; 18/24972-5 - Effects of early weaning on molecular markers during postnatal growth and development of intestinal, BP.TT

Abstract

The main functions of the gastrointestinal tract are the digestion and absorption of nutrients mediated by specialized cells of the epithelial tissue. The activity of development, renewal and regeneration is controlled by the niche of intestinal stem cells located at the base of the crypts, however it is still unknown how this niche of proliferative cells behaves in face of alterations in dietetic pattern. Considering breastfeeding as an important role in development of intestinal mucosa, early weaning may promote changes in the process of cell differentiation and proliferation. Recent studies have analyzed epigenetic patterns of intestinal epithelial cells and demonstrated that the plasticity of the cell is maintained by a permissive epigenomic state and the specification of cell line is directed by activation of signaling pathways and expression of factors and transcription. Therefore, the aim of this proposal is evaluate markers related to the process of cell proliferation, differentiation and growth factors of the intestinal epithelium as well as possible epigenetic variation observing the immediate and late effects of early weaning. During postnatal development, Wistar rats will be submitted to early weaning and small intestine will be collected at different ages for evaluation of gene and protein expression, detection and localization of important molecules for the establishment of the niche. The results obtained will be grouped and analyzed statistically. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA COSTA, ALINE VASQUES; GOES, CAROLINA PURCELL; GAMA, PATRICIA. Breastfeeding importance and its therapeutic potential against SARS-CoV-2. PHYSIOLOGICAL REPORTS, v. 9, n. 3, FEB 2021. (20/02888-2, 18/07782-8, 18/07409-5)

BARRETO E BARRETO, LAYLLA; RATTES, ISADORA C.; DA COSTA, ALINE V.; GAMA, PATRICIA. Paneth cells and their multiple functions. Cell Biology International, v. 46, n. 5, p. 10-pg., 2022-01-23. (18/05064-0, 18/07409-5, 18/07782-8)

DA COSTA, ALINE VASQUES; RATTES, ISADORA CAMPOS; GOES, CAROLINA PURCELL; LOBO, LARISSA HONDA GRECO; BARRETO, LAYLLA BARRETO E; GAMA, PATRICIA. Breastfeeding lifespan control of growth, maintenance, and metabolism of small intestinal epithelium. Journal of Cellular Physiology, v. N/A, p. 12-pg., 2023-08-09. (18/07409-5, 18/07782-8)

DA COSTA, ALINE VASQUES; RATTES, ISADORA CAMPOS; SILVA, KETHLEEN MESQUITA; MENDOZA RIGONATI, CRUZ ALBERTO; GAMA, PATRICIA. Effect of early weaning on proliferation and differentiation of intestinal mucosa. FASEB JOURNAL, v. 33, p. 2-pg., 2019-04-01. (18/07409-5, 18/07782-8)

TELES SILVA, MELISSA; MESQUITA DA SILVA, KETHLEEN; CAMPOS RATTES, ISADORA; MARIA AGOSTINI ZONTA, GIZELA; VASQUES DA COSTA, ALINE; GALVAO FIGUEREDO COSTA, RAQUEL; KAREN CORDEIRO NOGUEIRA, LUDIMILA; OGIAS, DANIELA; GAMA, PATRICIA. Immediate and Late Effects of Early Weaning on Rat Gastric Cell Differentiation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 1, JAN 1 2020. (11/17415-3, 12/10948-9, 14/21449-9, 16/21944-5, 18/24972-5, 18/07782-8)

SILVA, M. T.; DA SILVA, K. M.; OGIAS, D.; GAMA, P.. Trans-differentiation of gastric secretory cells during rat postnatal development: immediate and late effects of early weaning.. MOLECULAR BIOLOGY OF THE CELL, v. 29, n. 26, p. 1-pg., 2018-12-15. (18/07782-8)

MESQUITA DA SILVA, KETHLEEN; RATTES, ISADORA CAMPOS; PEREIRA, GIZELA MARIA AGOSTINI; GAMA, PATRICIA. Lifelong Adaptation of Gastric Cell Proliferation and Mucosa Structure to Early Weaning-Induced Effects. FRONTIERS IN PHYSIOLOGY, v. 12, SEP 13 2021. (14/21449-9, 18/05064-0, 18/07782-8)