Stereological evaluation of the surface density and total area surface of rumen and reticulum papillae

• Grant number: 18/11703-6
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: August 01, 2018
• End date: August 27, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Francisco Javier Hernandez Blazquez
• Grantee: Francisco Javier Hernandez Blazquez
• Visiting researcher: Augusto Coppi
• Visiting researcher institution: University of Surrey, England
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/50095-3 - Expression of proteins of cell communication and cell junctions in the digestive system of cattle fetuses, newborn calves and adult cattle, AP.TEM

Abstract

Studies with stereological design provide tools to obtain quantitative three-dimensional structural data of cells, tissues, organs or structures under study, as this tool has the sensitivity and accuracy necessary to detect small changes (Gundersen et al., 1988; BOYCE et al., 2010). Over the last few years, methods based on stereological design have become the cutting-edge methodology in quantitative histological analyzes. In addition, the application of these methods to the quantitative study of biological material contributed considerably to the understanding of the functional and pathological morphology of biological tissues (SHIMITZ and HOF, 2005).Historically, quantitative methods, generally referred to as two-dimensional methods, have been used for decades and are used to date. Stereology has been extensively used in studies of the central nervous system as a three-dimensional counting method to estimate the amount of neurons, vessels, synapses and other neural structures (Mura et al., 2004). In stereology each cell has an equal chance of being selected for counting. In this way, stereological techniques allow a deeper understanding of the neuropathological mechanisms of CNS diseases. In this project, Dr. Coppi will assist us in the stereological study of the rumen and reticulum surface, using surface calculation parameters based on stereology, a technique that is still little used in Brazil, and unprecedented in the study of rumen surface and reticulum. (AU)