Reproductive capacity, sex ratio, and offspring development of ethanol-preferring rats

• Grant number: 18/12354-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2018
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Francisco Eduardo Martinez
• Grantee: Francisco Eduardo Martinez
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 19/06365-7 - Reproductive capacity, sex ratio, and offspring development of ethanol-preferring rats, BP.TT; 18/24725-8 - Reproductive capacity, sex ratio, and offspring development of ethanol-preferring rats, BP.TT

Abstract

Alcoholism is a disease with inherited factors related to ethanol abuse and dependence. Teratogenesis, behavioral and cognitive alterations, and decreased fertility due to use of ethanol. We will evaluate whether the parameters of reproduction and development of alcoholic rats predisposed to ethanol consumption differ from non-consuming healthy rats and whether the variables are dose-dependent. Male and female rats will be divided into control (Wistar - C) and experimental A (Wistar UChA) and B (Wistar UChB) groups, treated with feed and water ad libitum, and 10% ethanol diluted in water in the experimental groups from of 65 days of life. Pairs will be formed into adulthood for mating. Groups C, A and B will have their correlated data to evaluate whether the reproductive capacity of parents and the sex ratio of offspring between healthy (C) and sick (A and B) differ. For the analysis of the reproductive parameters of offspring will be used descendants of groups B and C, monitored from birth. The standard of ethanol consumption between males and females will also be tested. The statistical significance in the experiments will be calculated by unpaired t test and ANOVA (p <0.05). Data will be represented as averages and standard deviation. GraphPad Prism will be used for graphing. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIORAVANTE, VANESSA CAROLINE; GODOI, ALANA REZENDE; DE BARROS CAMARGO, VICTORIA MOKARZEL; DO NASCIMENTO, RENATA STEFFANY; FELIPE PINHEIRO, PATRICIA FERNANDA; MARTINEZ, FRANCISCO EDUARDO. Parents ethanol use impairs ethanol-naive offspring development and reproduction. Reproduction, v. 161, n. 2, p. 195-204, FEB 2021. (18/12354-5)

FIORAVANTE, VANESSA CAROLINE; GODOI, ALANA REZENDE; DE BARROS CAMARGO, VICTORIA MOKARZEL; FELIPE PINHEIRO, PATRICIA FERNANDA; MARTINEZ, MARCELO; PADOVANI, CARLOS ROBERTO; MARTINEZ, FRANCISCO EDUARDO. Low and high postpubertal ethanol use: damage on adulthood reproduction and offspring. REPRODUCTION AND FERTILITY, v. 3, n. 3, p. 12-pg., 2022-07-01. (18/12354-5)