Replication and cellular effects of rhinovirus in lymphoid tissues.

• Grant number: 18/25605-6
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2019
• End date: August 31, 2021
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Eurico de Arruda Neto
• Grantee: Eurico de Arruda Neto
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Rhinovirus (VR) is the infectious agent that most frequently affects the human species, causing acute respiratory infections (ARI), and is often associated with exacerbations of asthma and chronic bronchitis / emphysema, as well as secondary complications such as sinusitis and otitis media. Studies done by our group have shown that RV often infects tonsils and is excreted in nasopharyngeal secretions of patients with chronic tonsillar hypertrophy, even in the absence of ARI symptoms. In these tissues, RV infects both epithelial cells and CD4 and B-T lymphocytes. In addition to palatine and adenoid tonsils, preliminary data recently obtained by us showed that RV also infects lymphocytes in lymph nodes and spleen, suggesting that the infection has a systemic character, an original information that must be investigated in its consequences, including the possibility of persistence of RV in prolonged infection. Therefore, the experiments presently proposed aim to investigate RV replication and its effects on lymphoid cells. The overall goals are to confirm the systemic character of RV infection by studying tissue samples routinely obtained at necropsies; to compare epithelial and/or fibroblast cells with lympho-mononuclear ones with regard to RV replication and consequent molecular and cellular damages; to evaluate the activity of gene products involved in inflammation and antiviral response in primary cultures of epithelial cells and/or fibroblasts compared to lympho-mononuclear cells; and to verify the viral genetic diversity, the presence of defective viral genomes, and the viral population dynamics in lympho-hematopoietic cells in an experimental mouse model. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, RONALDO B.; CASTRO, ITALO A.; PONTELLI, MARJORIE; SOUZA, JULIANO P.; LIMA, THAIS M.; MELO, STELLA REZENDE; ZEN SIQUEIRA, JOAO PAULO; CAETANO, MAICON HENRIQUE; ARRUDA, EURICO; GOTTARDO DE ALMEIDA, MARGARETE TERESA. SARS-CoV-2 Inactivation by Ozonated Water: A Preliminary Alternative for Environmental Disinfection. OZONE-SCIENCE & ENGINEERING, v. 43, n. 2, p. 108-111, MAR 4 2021. (18/25605-6)