Effects of caffeine exposure on mitophagic pathways: actions over myogenic progenitor cells differentiation

• Grant number: 19/12236-5
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2019
• End date: May 31, 2022
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Igor Luchini Baptista
• Grantee: Igor Luchini Baptista
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

The skeletal muscle regenerative process is critical for tissue functional recovery after any lesion stimuli, micro-injuries or large injuries. Thus, the ability to regenerate allows the skeletal muscle to keep the homeostasis and, consequently, the whole body's health. In our previous study (under revision), we have demonstrated that in Parkin's absence, myogenic progenitor cells present a reduced autophagic process with delayed skeletal muscle regeneration. The caffeine has been associated with the prevention of neurotoxicity related to Parkinson. However, few studies have approached the caffeine effects over myogenic progenitor cells. Our hypothesis is caffeine could act as a therapeutic agent by regulating the mitochondrial population since it has been demonstrated that caffeine can induce autophagy in several cell types and mitochondrial biogenesis in muscle fibers. Thus, our main objective is to investigate the effects of caffeine over the differentiation process of myogenic cells in vitro, and over skeletal muscle regeneration on wild type (WT) and Parkin knockout (Parkin-/-) animals. To improve our knowledge about caffeine metabolism, we also aim to verify whether Parkin-/- present a deficient capacity to absolve and to metabolize caffeine, as suggested to humans with Park2 mutations. That information will be relevant to indicate new ways of caffeine action on skeletal muscle tissue, which can be used further to myopathies treatment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NEMEZIO, KLEINER; YAMAGUCHI, GUILHERME DE CARVALHO; BOITO RAMKRAPES, ANA PAULA; SCHULZ, MARIANE LEICHSENRING; BAPTISTA, IGOR LUCHINI; RIANI, LUIZ AUGUSTO; GONCALVES, LIVIA DE SOUZA; SALE, CRAIG; GENNARI DE MEDEIROS, MARISA HELENA; GUALANO, BRUNO; et al. The role of chronic muscle (in)activity on carnosine homeostasis: a study with spinal cord-injured athletes. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 320, n. 6, p. R824-R832, JUN 2021. (14/11948-8, 19/25032-9, 17/13552-2, 13/07937-8, 15/23176-2, 13/14746-4, 19/12236-5)

ESTECA, V, MARCOS; SEVERINO, MATHEUS B.; SILVESTRE, JOAO G.; DOS SANTOS, GUSTAVO PALMEIRA; TAMBORLIN, LETICIA; LUCHESSI, AUGUSTO D.; MORISCOT, ANSELMO S.; GUSTAFSSON, ASA B.; BAPTISTA, IGOR L.. Loss of Parkin Results in Altered Muscle Stem Cell Differentiation during Regeneration. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 21, NOV 2020. (16/25876-4, 19/12236-5)