Development of colorimetric kits for quantification of porcine circoviruses 2 (PCV-2) and SARS-CoV2 using gold nanoparticles

• Grant number: 19/18581-6
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2020
• End date: March 31, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: João Pessoa Araújo Junior
• Grantee: João Pessoa Araújo Junior
• Host Institution: Instituto de Biotecnologia (IBTEC). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu
• Associated researchers: Alexandre Naime Barbosa ; Caroline Rodrigues Basso ; Rejane Maria Tommasini Grotto ; Taís Fukuta da Cruz ; Valber de Albuquerque Pedrosa

Abstract

Until the morning of 03/31/2020, 719,700 confirmed cases of SARS-Cov-2 were registered worldwide, causing a collapse in the health system of countries such as Italy, Iran and Spain. Brazil has recorded 4,661 confirmed cases, with 165 deaths. The transmission of the virus occurs mainly by contact with secretions and infected people, causing fever, cough, dyspnoea, myalgia or fatigue, sputum production, headache, hemoptysis and diarrhea. Diagnosis occurs after medical evaluation and laboratory tests such as RT-qPCR. However, these methodologies present as limitations a high cost per analysis of the samples, besides needing specialized professionals and specific equipment. Therefore, it is necessary to develop a new reliable and low-cost methodology for a fast and inexpensive quantitative diagnosis. Biosensors have been highlighted in the technological area as an analytical tool that can identify the presence of pathogens (antigens or nucleic acid) using as base gold nanoparticles. Therefore, the objective of the present project is to develop and standardize colorimetric kits for the identification of SARS-CoV-2 and viral RNA using gold nanoparticles with deposition of antibodies or probes which, when connected to their respective target, will provide an immediate change of color. This is a new technique that will provide a quick, simple and low-cost diagnosis compared to the usual methodologies (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BASSO, CAROLINE RODRIGUES; YAMAKAWA, ANA CAROLINA; CRUZ, TAIS FUKUTA; PEDROSA, VALBER ALBUQUERQUE; MAGRO, MASSIMILIANO; VIANELLO, FABIO; ARAUJO JUNIOR, JOAO PESSOA. Colorimetric Kit for Rapid Porcine Circovirus 2 (PCV-2) Diagnosis. PATHOGENS, v. 11, n. 5, p. 15-pg., 2022-05-01. (19/20573-1, 19/18581-6)

DA COSTA, VIVALDO GOMES; GOMES, ANA JULIA CHAVES; BITTAR, CINTIA; GERALDINI, DAYLA BOTT; DA CONCEICAO, PAMELA JOYCE PREVIDELLI; CABRAL, AGATA SILVA; CARVALHO, TAMARA; BISELLI, JOICE MATOS; PROVAZZI, PAOLA JOCELAN SCARIN; CAMPOS, GUILHERME RODRIGUES FERNANDES; et al. Burden of Influenza and Respiratory Syncytial Viruses in Suspected COVID-19 Patients: A Cross-Sectional and Meta-Analysis Study. Viruses-Basel, v. 15, n. 3, p. 16-pg., 2023-03-01. (20/07419-0, 19/18581-6, 21/00603-3)

POSSEBON, FABIO SOSSAI; ULLMANN, LEILA SABRINA; MALOSSI, CAMILA DANTAS; MIODUTZKI, GABRIELLE THAIS; DA SILVA, EVELYN CRISTINE; MACHADO, EDUARDO FERREIRA; BRAGA, IOLANDA SIMOES; PELAQUIM, ISADORA FERNANDA; ARAUJO JR, JOAO PESSOA. fast and cheap in-house magnetic bead RNA extraction method for COVID-19 diagnosi. Journal of Virological Methods, v. 300, FEB 2022. (19/18581-6)

LAMARCA, ALESSANDRA P. P.; DE SOUZA, UERIC JOSE BORGES; MOREIRA, FILIPE ROMERO REBELLO; DE ALMEIDA, LUIZ G. P.; DE MENEZES, MARIANE TALON; DE SOUZA, ADRIELI BARBOZA; FERREIRA, ALESSANDRO CLAYTON DE SOUZA; GERBER, ALEXANDRA L.; DE LIMA, ALINE B.; GUIMARAES, ANA PAULA DE C.; et al. The Omicron Lineages BA.1 and BA.2 (Betacoronavirus SARS-CoV-2) Have Repeatedly Entered Brazil through a Single Dispersal Hub. Viruses-Basel, v. 15, n. 4, p. 13-pg., 2023-04-01. (19/18581-6)

BASSO, CAROLINE RODRIGUES; MALOSSI, CAMILA DANTAS; HAISI, AMANDA; PEDROSA, VALBER DE ALBUQUERQUE; BARBOSA, ALEXANDRE NAIME; GROTTO, REJANE TOMMASINI; ARAUJO JUNIOR, JOAO PESSOA. Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance. ANALYTICAL METHODS, v. 13, n. 29, p. 3297-3306, AUG 7 2021. (19/20573-1, 19/18581-6)

Filed patent(s) as a result of this research project