COVID-19: selection of specific inhibitors for main protease Mpro of SARS-CoV-2 by phage display

• Grant number: 20/05451-4
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2020
• End date: December 31, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Aparecida Sadae Tanaka
• Grantee: Aparecida Sadae Tanaka
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 19/03779-5 - Use of phage display as a tool in the diagnosis and control of diseases transmitted by hematophagous vectors, AP.TEM
• Associated scholarship(s): 21/12804-3 - COVID-19: selection of specific inhibitors for the main protease Mpro from SARS-CoV-2 by phage display, BP.PD; 20/09222-0 - COVID-19: selection of specific inhibitors for the main protease of SARS-COV-2 Mpro by phage display, BP.PD

Abstract

The severe acute respiratory syndrome COVID-19 caused by the coronavirus SARS-CoV-2 is an emerging and highly contagious disease, with which the world is experiencing a pandemic from the end of 2019 to the present day, which has led several countries to bankruptcy public health service, and soon Brazil will reach this situation. Today (13/04/2020) there are already at least 22300 people infected and 1230 deaths in the country. Worldwide, the number of deaths exceeds 116,000. The virus is advancing rapidly around the world, and everyone who can is looking for alternatives to contain it, so far, we have no medicine, vaccine or other method of control other than social detachment. In order to contribute to this search for scientific knowledge in developing of specific anti-SARS-CoV-2 therapies, the molecular mechanisms that promote and sustain viral infection must be defined. An important therapeutic target among coronaviruses is the main protease Mpro (or 3CLpro), due to its essential role in processing the polyproteins that are translated from viral RNA and enabling the formation of the viral particle. Thus, the purpose of this work is to identify specific inhibitors for the main Mpro protease of SARS-CoV-2 through the phage display technique using peptide libraries and protein inhibitors. (AU)