Spatial analysis and molecular research to Coxiella burnetii in bovine milk collected from bulk tanks from small farms

• Grant number: 20/09409-2
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2020
• End date: November 30, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Simone Baldini Lucheis
• Grantee: Simone Baldini Lucheis
• Host Institution: APTA Regional. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated researchers: Diego Borin Nobrega ; Maria Izabel Merino de Medeiros ; Virgínia Bodelão Richini Pereira

Abstract

Coxiella burnetii (C. burnetii) is a gram-negative bacterium that causes a cosmopolitan zoonosis called Q fever. It infects a range of animals, with the group of large ruminants (cattle) being the largest reservoir. It is known to cause gestational disorders in animals, such as abortion, stillbirths and premature births, in addition to causing infertility, generating a great economic impact. Infection in humans occurs through the inhalation of aerosols containing the bacteria, derived from the excrement, milk or childbirth products of these animals. Therefore, the objective of this work is to research C. burnetii in 102 milk samples from lactating cows, using milk from bulk tanks of small family farms in different municipalities in the Midwest region of São Paulo, using the molecular techniques of conventional Polymerase Chain Reaction (PCR), Nested PCR and real-time PCR (qPCR), as well as performing genetic sequencing. Subsequently, a geographic analysis of the distribution of positive cases diagnosed in the different regions will be carried out. This study is justified, considering the importance of this zoonosis in public health and few studies in the literature about the transmission of C. burnetii from bovine milk and from bulk tanks. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAPPAROLI, DIANA; BERTOZZO, THAINA VALENTE; ALEXANDRINO, MARCELA; SANCHES, DANIELA FILADELFO; AIRES, ISABELLA NEVES; MANZINI, SUZANE; MERINO DE MEDEIROS, MARIA IZABEL; KUROKAWA, CILMERY SUEMI; DOS SANTOS, RODRIGO MATTOS; LUCHEIS, SIMONE BALDINI. Commercially acquired acai pulps contamination by Trypanosoma cruzi. International Journal of Food Microbiology, v. 363, p. 5-pg., 2022-02-16. (20/09409-2)