Electronic cigarette: cytodiagnosis and salivary metabolome and biophotonic

• Grant number: 20/10362-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2021
• End date: October 31, 2023
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Janete Dias Almeida
• Grantee: Janete Dias Almeida
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• Associated researchers: Herculano da Silva Martinho ; Maria Anita Mendes ; Mario Pérez-Sayáns Garcia ; Mônica Ghislaine Oliveira Alves
• Associated research grant(s): 24/07296-7 - 10th International Symposium on Advances in Oral Cancer entitled "20 years of Advances in Oral Cancer", AR.EXT

Abstract

The electronic cigarette (e-cig) was developed to promote and facilitate smoking cessation, however, the relationship of risks and benefits as well as the effects of e-cig on the oral mucosa are unknown. The objective of this study is to evaluate the effect of using e-cig on saliva and the oral mucosa of its users. Two groups will be composed: Electronic Cigarette Group (GCE) - 48 regular and exclusive e-cig users; Control group (GCO) - 48 non-smoking and non-e-cig users, matched in gender and age to the GCE. All participants will be examined. Afterwards, unstimulated saliva will be collected for the evaluation of the salivary metabolome. In addition, 2 ml of the liquid present in each participant's e-cig will be collected for the evaluation of their composites. Both analyzes will be done by means of gas chromatography coupled to the mass spectrometer (GC-MS). Saliva will also be submitted to vibrational spectroscopy analysis (Raman dispersive, FT-Raman, FTIR). The cytological smear will be done on the left side of the tongue for citochemical evaluation and, on the right side, for gene expression evaluation. The exfoliative cytology will be evaluated by the Papanicolaou method (cytomorphological evaluation - inflammation and cellular maturation), Silver staining (evaluation of nucleolar organizer regions - AgNORs) and by qRT-PCR (CYP1A1, CYP1A1, CYP2A6, CYP2E1, L1B, IL6, CXCL8, TNF, CCL2, PTGS2, KRT1, KRT5, KRT13, KRT15, TP16, TP21 and TP53). The data will be treated by Student t-test (parametric data) or Mann-Whitney test (non-parametric data). For metabolic analysis, will be performed in addition, the principal component analysis (PCA) and ROC curve. The associations between variables and clinical data will be explored using Spearman's correlation coefficient. For all analyzes, a significance level of 5% will be adopted. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GREENFIELD, ELLEN; ALVES, MARIANA DE SA; RODRIGUES, FERNANDA; NOGUEIRA, JULIA OLIVEIRA; DA SILVA, LETICIA FERRARI; DE JESUS, HENRIQUE PEREIRA; CAVALCANTI, DESIREE ROSA; DO CARMO CARVALHO, BRUNA FERNANDES; ALMEIDA, JANETE DIAS; MENDES, MARIA ANITA; et al. Preliminary Findings on the Salivary Metabolome of Hookah and Cigarette Smokers. ACS OMEGA, v. 8, n. 40, p. 11-pg., 2023-09-30. (20/10322-9, 18/23248-1, 20/10362-0)

CARVALHO, BRUNA FERNANDES DO CARMO; ALVES, MONICA GHISLAINE OLIVEIRA; MARQUES, SANDRA SILVA; LOPES, MARCIO AJUDARTE; PEREZ-SAYANS GARCIA, MARIO; ALMEIDA, JANETE DIAS. Dentists, are you ready to deal with the "smokers of the future"?. HUMAN & EXPERIMENTAL TOXICOLOGY, v. 41, p. 2-pg., 2022-05-01. (20/10322-9, 20/10362-0)