Bioprinted human skin equivalent validation 3DBSkin full-thickness bioprinted model

• Grant number: 21/06621-3
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: March 01, 2022
• End date: August 31, 2023
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Agreement: FINEP - PIPE/PAPPE Grant
• Principal Investigator: Gabriela Gomes Cardoso Gastaldi
• Grantee: Gabriela Gomes Cardoso Gastaldi
• Company: Soluções em Biotecnologia 3D - Elaboração de Projetos Ltda
• CNAE: Pesquisa e desenvolvimento experimental em ciências físicas e naturais ; Atividades de apoio à gestão de saúde ; Atividades de atenção à saúde humana não especificadas anteriormente
• City: Campinas
• Associated researchers: Ana Carolina Migliorini Figueira

Abstract

There is a global movement for the improvement of in vitro testing aiming to substitute the use of animals in security evaluation essays. Since 2019 the resolution Resolução Normativa do Conselho Nacional de Controle de Experimentação Animal (Concea) forbids testing for efficacy and security evaluation using animals. The industry uses a great amount of tests in the process of evaluation and security, starting with well established in vitro testing. The development of in vitro tridimensional models, such as skin artificial models, possible to be replicated, functional and scalable is a challenge in the field of the tissue engineering. This project aims to validate a bioprinted human skin of the startup 3D Biotechnology Solutions intra and inter laboratories. The 3DBSkin is a dermal-epidermal equivalent developed using 3D bioprinting technology based on collagen type I bioink, fibroblasts, primary keratinocytes and, more recently, melanocytes, focusing on a pigmented skin model. Differently from the manual models commercialized today, the proposed model has a greater reproducibility and scalability. Issues regarding better barrier function properties are going to be evaluated in the project. This model is to be validated intra laboratorial, in the startup 3D Biotechnology Solutions and inter laboratorial in two centers that are part of the Rede Nacional de Métodos Alternativos (Renama) related to animal use in research. The Centro Nacional de pesquisa em Energia e Materiais - CNPEM - LNBio and the enterprise Kosmoscience are testing for corrosion and irritation according to the OCDE guides 431 and 439, respectively, that normatize the requisites internationally. Keywords: full-thickness bioprinted skin model, alternative human skin, OCDE validation, cosmetics, in vitro equivalents (AU)