Fungi of the genera Trichoderma and Purpureocillium in soybean plants: evaluation of endophytic colonization, growth promotion, and influence on the associated microbiome

• Grant number: 21/10821-8
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: May 01, 2022
• End date: April 30, 2024
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Everlon Cid Rigobelo
• Grantee: Everlon Cid Rigobelo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 22/10810-9 - Trichoderma and Purpureocillium in soybean plants: evaluation of endophytic colonization, Promoting growth and influencing the associated microbiome, BP.TT

Abstract

Soybean is one of Brazil's main export products. Its importance to the national economy is reflected in the fact that it occupies most of the country's arable land, over 35 million hectares. The INTACTA RR2 PROTM variety, resistant to glyphosate and modified for the production of Bt toxins that confer insecticidal action against various crop pests, is widely used in the country. Currently, the development of new cultivars is constant, and it is carried out in the search for greater productivity and reduction of production costs. Allied to this strategy, the adoption of biological management techniques is growing and represents the future to reduce the use of agricultural inputs, including pesticides and fertilizers, in the search for more balanced and sustainable agriculture, especially in the cultivation of soybean. The use of fungi in biological pest management practices is among the most widespread. New perspectives regarding its interaction with plants, specifically on the occurrence of an endophytic relationship capable of providing several benefits, have been the target of new research, which still lacks in planta and field studies. In addition, the influence of the presence of bioinoculants on the microbiome associated with plants is a practically unexplored aspect, besides being very important for understanding the ecological aspects that can favor the growth and productivity of crops. Thus, the present study aims to characterize the potential of endophytic colonization of soybean plants of the INTACTA RR2 PROTM variety by Trichoderma sp. LSM122 and Purpureocillium lilacinum LSM65 by transforming them for the expression of fluorescent proteins. These fungi already have their growth-promoting potential described in vitro and, in this study, it is expected to evaluate their in plants effect, both in greenhouse and field conditions, in addition to characterizing the influence of their presence on the composition of the microbiome associated with soybean plants. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ANDRADE, LUANA ALVES; SANTOS, CARLOS HENRIQUE BARBOSA; FREZARIN, EDVAN TECIANO; SALES, LUZIANE RAMOS; RIGOBELO, EVERLON CID. Plant Growth-Promoting Rhizobacteria for Sustainable Agricultural Production. MICROORGANISMS, v. 11, n. 4, p. 16-pg., 2023-04-01. (21/10821-8)