Pharmagenetic study and PK/PD after application of infiltrative lidocaine associated or not with epinephrine in saliva samples by LC MS/MS

• Grant number: 22/11140-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2023
• End date: March 31, 2026
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Carlos Ferreira dos Santos
• Grantee: Carlos Ferreira dos Santos
• Host Institution: Hospital de Reabilitação de Anomalias Craniofaciais (HRAC). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Adriana Maria Calvo ; Thiago José Dionísio
• Associated research grant(s): 25/00054-0 - 2025 IADR/PER General Session & Exhibition, AR.EXT
• Associated scholarship(s): 24/17291-2 - Development of an analytical method for lidocaine and metabolite determination by LCMS/MS in saliva samples., BP.TT

Abstract

The individualization of drug prescription has increasingly become the focus of current research in pharmacology so that the adverse effects of drugs are minimized whenever possible. Some studies in the current literature show the relationship that cytochrome P450 polymorphisms, more specifically CYP3A4 and CYP1A2, exert on the metabolism of lidocaine into its main metabolite, monoethyl-glycinexylidide (MEGX). Lidocaine is the most used local anesthetic in dentistry worldwide, being one of the oldest sodium channel blockers on the market and considered the safest amide local anesthetic. Even so, it can cause some side effects on the cardiovascular system and the central nervous system, especially when accidental administration occurs directly into a blood vessel, and the association with vasoconstrictors, especially epinephrine, is a strategy to minimize these effects. Clinical data will be collected from 20 patients, research participants, who will attend two different dental appointments, according to the protocol, for scaling and crown-radicular polishing, sulcular infiltrative injection in the region of maxillary molars of a cartridge of lidocaine associated or not with 1:100,000 epinephrine. For the pharmacokinetic (PK) study, saliva samples will be collected. For the pharmacodynamic parameters (PD), variations in blood pressure, oximetry, and heart rate will be evaluated during the procedure, in addition to the beginning and duration of local anesthesia in soft tissues. Altogether, data will be analyzed for CYP3A4 and CYP1A2 polymorphisms and their relationship with individual patient responses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, GABRIELA MORAES; DIONISIO, THIAGO JOSE; SIQUEIRA-SANDRIN, VIVIANE SILVA; FERRARI, LETICIA ALVES DE LIMA; COLOMBINI-ISHIKIRIAMA, BELLA LUNA; FARIA, FLAVIO AUGUSTO CARDOSO; SANTOS, CARLOS FERREIRA; CALVO, ADRIANA MARIA. Liquid Chromatography-Tandem Mass Spectrometry Method for Detection and Quantification of Meloxicam and 5 '-Carboxymeloxicam in Oral Fluid Samples. METABOLITES, v. 13, n. 6, p. 14-pg., 2023-06-01. (17/12725-0, 22/11140-7)