Study of the impact of metallic nanoparticles produced with organic waste, methyl aminolevulinate and gamma-aminobutyric acid in green nanopriming

• Grant number: 22/14030-8
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2023
• End date: August 31, 2025
• Field of knowledge: Physical Sciences and Mathematics - Physics
• Principal Investigator: Lilia Coronato Courrol
• Grantee: Lilia Coronato Courrol
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Marcia Regina Franzolin
• Associated scholarship(s): 25/03970-8 - Sustainable Nanotechnology: Use of Selenium and Silver-Copper Nanoparticles Synthesized by Photoreduction with Banana Peel for Controlling Panama Disease in Banana Plants, BP.IC

Abstract

The sustainable growth of agriculture depends on innovative techniques such as those based on nanotechnology. This project aims to evaluate the potential impact of green silver, copper, and silver-copper nanoparticles (NPs) on the nanopriming of wheat and sunflower seeds. Nanopriming consists of conditioning seeds in a solution containing NPs for a certain period. This technique helps germination and seed growth by modulating biochemical pathways and balancing reactive oxygen species and plant growth hormones, resulting in increased resistance to biotic and abiotic stress. In this sense, nanopriming reduces pesticides and fertilizers, contributing to sustainable agricultural practices. Green nanopriming uses nanoparticles synthesized by green chemistry with elements that are non-toxic to the environment. In this project, NPs will be produced with organic waste extracts (banana and pineapple peels) and with plant growth regulators, a derivative of aminolevulinic acid (ALA), methyl aminolevulinate (MALA), and gamma-aminobutyric acid (GABA). The nanoparticles will be characterized by UV-Vis, FTIR, zeta potential, X-ray diffraction, high-performance liquid chromatography, polydispersity index, electron microscopy imaging, and antimicrobial properties. Seeds will be conditioned in NP solutions, and their germination and growth will be monitored over time. The effect of nanopriming under different 1) priming conditions: temperature, lighting, duration, and 2) NPs parameters: nature, shape, size, and concentration of NPs will be evaluated from the germination percentage, vigor index, and chlorophyll concentration of the seedlings. In addition, the activity of antioxidant enzymes such as catalase (CAT), peroxidase (POD), glutathione reductase (GR), and superoxide dismutase (SOD) will be determined. The generation of reactive oxygen species (ROS) will be evaluated in the nanopriming process. We will thoroughly investigate the optical properties of chlorophyll to identify possible changes in the fluorescence spectrum or decay time due to the presence of silver and copper ions or nanoparticles in seedlings. With this project, we want to understand better the mechanisms involved in NPs synthesis and determine the best NPs to be used in green nanopriming to contribute to more sustainable agriculture. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, ISABELA SANTOS; ZULUAGA, NORMA LUCIA BURITICA; CUCCOVIA, IOLANDA MIDEA; FRANZOLIN, MARCIA REGINA; DOS SANTOS, BEATRIZ FUZINATO; WODTKE, FELIPE; DARBEM, MARIANA P.; DOS SANTOS, ALCINDO A.; COURROL, LILIA CORONATO. Interactions between gamma-aminobutyric capped silver nanoparticles and large unilamellar vesicles (LUVs) and their antimicrobial activities. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 101, p. 7-pg., 2024-09-11. (22/14030-8, 14/50983-3)

LOPES, ISABELA SANTOS; SANTOS, NOEMY RODRIGUES; DA SILVA, GIOVANA DIAS; DOS SANTOS, LEONARDO CAMARGO; POTIENS, MARIA DA PENHA ALBUQUERQUE; VIEIRA, DANIEL PEREZ; COURROL, LILIA CORONATO. Evaluation of low-kV energy X-ray radiation effect on breast cancer cells incubated with gold nanoparticles capped with aminolevulinic acid, methyl aminolevulinate, and gamma-aminobutyric acid. Radiation Physics and Chemistry, v. 218, p. 8-pg., 2024-02-06. (22/14030-8, 14/50983-3)

LOPES, ISABELA SANTOS; SOARES, JULLIO KENNEDY CASTRO; DE MEDEIROS, LIVIA SOMAN; COURROL, LILIA CORONATO. Evaluation of ALA-capped silver, copper, and silver-copper nanoparticles for controlling fungal plant pathogens. Microbial Pathogenesis, v. 191, p. 10-pg., 2024-05-07. (22/14030-8, 20/08270-0, 22/14896-5)

LOPES, ISABELA SANTOS; VITAL, VITOR GONCALVES; PANTAROTTO, SUZAN VASCONCELOS; COURROL, LILIA CORONATO. The Synergistic Effects of Gamma-Aminobutyric Acid Silver Nanoparticles and Light in Antimicrobial Photodynamic Inactivation. NANOPHOTONICS X, v. 12991, p. 9-pg., 2024-01-01. (22/14030-8)

TEIXEIRA, BRUNA HENRIQUE; GONCALVES, KARINA DE OLIVEIRA; VIEIRA, DANIEL PEREZ; COURROL, LILIA CORONATO. Synergizing Immune Balance: Curcumin Gold Nanoparticles and Ultrasound Irradiation for Macrophage Down-Regulation. APPLIEDCHEM, v. 4, n. 1, p. 16-pg., 2024-03-06. (14/50983-3, 22/14030-8)

LOPES, ISABELA SANTOS; DE MORAES, BIANCA NATASHA OLIVEIRA; BARRETO, SUSANA DE SOUZA; LE JONCOUR, LEA; COUTEAU, CHRISTOPHE; FRANZOLIN, MARCIA REGINA; COURROL, LILIA CORONATO. Green synthesis of antimicrobial silver-copper nanoparticles using Banana and pineapple peel extracts: A sustainable approach for biomaterial sterilization. Materials Chemistry and Physics, v. 333, p. 13-pg., 2025-01-11. (22/14030-8)

LOPES, ISABELA SANTOS; YEE, MARCIO; TATUMI, SONIA HATSUE; VITAL, VITOR GONCALVES; DE LIMA, LUCAS FURLANETO; DE VASCONCELLOS, SUZAN PANTAROTO; COURROL, LILIA CORONATO. GABA functionalized gold, silver, and copper nanoparticles: Synthesis, characterization and potential applications. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 92, p. 8-pg., 2024-01-20. (22/14030-8)