Glycosaminoglycans and the cross-talk cell and extracellular matrix: a translational approach

• Grant number: 23/07464-4
• Support Opportunities: Research Projects - Thematic Grants
• Start date: August 01, 2024
• End date: July 31, 2029
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Helena Bonciani Nader
• Grantee: Helena Bonciani Nader
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Fábio Dupart Nascimento
• Associated researchers: Caio Vinicius Saito Regatieri ; Carla Cristina Lopes de Azevedo ; Giselle Zenker Justo ; Juliana Luporini Dreyfuss Regatieri ; Leny Toma ; Maria Aparecida da Silva Pinhal
• Associated research grant(s): 24/15793-0 - Multi-user Equipment approved in grant 2023/07464-4: TRX1200, NMR RF transceiver unit, AP.EMU; 24/15795-3 - Multi-user equipment approved in grant 2023/07464-4: preparative ultracentrifuge, AP.EMU
• Associated scholarship(s): 24/20473-5 - The role of Syndecan 4 in the cellular traffic and extracellular matrix remodeling, BP.PD; 24/21930-0 - "Combined effect of anti-VEGF and chemically modified heparin. Role in the inhibition of angiogenesis in ocular diseases, an in vitro study.", BP.IC; 24/20700-1 - Heparins and heparinoids: potencial antiinflammatory, antiangiogenic an antitumor molecules, BP.DD

Abstract

The extracellular matrix (ECM) is constantly remodeling in response to various extracellular and intracellular stimuli, and the way in which these signals are transmitted, captured and interpreted by cells, determines and differentiates the fate of normal or pathological remodeling. Response to stimuli is a hallmark of the definition of life. Communication between cells is essential and is profoundly influenced by the ECM. In this context, the ECM and its remodeling, as well as the interactions between its different components constitute a research area at the frontier of knowledge. The present proposal encompasses three main approaches: 1) Systematic characterization of the structure of glycosaminoglycans and proteoglycans of the matrix and cell surface; 2) Study of ECM functions using biological models in vitro, in vivo and ex vivo; 3) Cell response to ECM variations. A methodological arsenal involving state-of-the-art techniques from glycobiology, cellular, molecular and structural biology, bioengineering and translational science will be used to respond to these hypotheses. (AU)