Multi-user equipment approved in grant 23/07482-2: microscope Celldiscoverer 7 KMAT

• Grant number: 24/08997-9
• Support Opportunities: Multi-user Equipment Program
• Start date: September 01, 2024
• End date: August 31, 2031
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Niels Olsen Saraiva Câmara
• Grantee: Niels Olsen Saraiva Câmara
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 23/07482-2 - Sensing extra and intracellular stressors by renal and immune cells: new insights into signal reception and transduction, and their relevance for understanding renal diseases, AP.TEM

Abstract

Chronic kidney diseases are still a leading cause of death worldwide. Thus, the need for new treatment approaches is urgent. Here, we aim to unravel new sensing mechanisms for organelles on renal and immune cells and how the external and internal perception of unbalance is involved in responses which triggers injury, influences repair, and promotes renal tissue fibrosis. We hypothesize that organelles have a central role on sensing and transducing signals from different source. To do that, we intend to use in vivo (mouse and zebrafish models), in vitro (cell culture, organoids), in silico (scRNAseq, omics) and ex-vivo (Human samples) approaches, employing commonly used experimental methods to evaluate cell function as well as cutting-edge technologies (e.g., Organ-on-chip, single cell RNA sequencing - scRNAseq; Forster resonance energy transfer-FRET; CRISPR-Cas9; the newly developed imaging technique MINFLUX). We believe that understanding the new functions attributed to organelles could be a potential new strategy to treat kidney diseases. (AU)