Using RNA Interference Technology to Control Insect Vectors and Geminiviruses

• Grant number: 24/15503-2
• Support Opportunities: Research Program on Global Climate Change - Regular Grants
• Start date: June 01, 2025
• End date: May 31, 2028
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Agreement: National Natural Science Foundation of China (NSFC)
• Principal Investigator: Gabriel Madoglio Favara
• Grantee: Gabriel Madoglio Favara
• Principal researcher abroad: Yi Xu
• Institution abroad: Nanjing Agricultural University , China
• Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil
• City of the host institution: Ilha Solteira
• Associated researchers: Felipe Franco de Oliveira ; Jorge Alberto Marques Rezende ; Washington Luis da Silva
• Associated scholarship(s): 25/08640-6 - Topical application of dsRNA to control tomato severe rugose virus (ToSRV) on tomato plants, BP.MS; 25/08915-5 - Topical application of dsRNA to control Bemisia tabaci MEAM1 on tomato plants, BP.MS

Abstract

Bemisia tabaci is a significant global agricultural pest, primarily due to its role as a vector for plant viruses. Among these, tomato severe rugose virus (ToSRV) is one of the most prevalent begomoviruses affecting tomato crops in Brazil. In conditions of high inoculum and vector pressure, ToSRV can cause damage, with yield losses reaching up to 100%. Current control strategies predominantly rely on the frequent application of insecticides targeting B. tabaci, underscoring the need for alternative management approaches. RNA interference (RNAi) has emerged as a promising alternative for managing pests, insect vectors, and plant pathogens. This mechanism is triggered by the presence of double-stranded RNA (dsRNA) within the cell, leading to the sequence-specific degradation of target RNAs that are homologous to the dsRNA. RNAi offers a sustainable and innovative solution for pest and pathogen control, aligning with the principles of modern agriculture that emphasize environmental protection. Unlike conventional methods, RNAi operates with high specificity, targeting only essential genes in the pest or pathogen, thus minimizing collateral damage to non-target organisms and ecosystems. This technology also has the potential to reduce dependence on chemical pesticides, which are associated with environmental contamination and the development of resistance in pest populations. The primary objective of this project is to develop a dsRNA-based system to induce RNAi for the control of B. tabaci and ToSRV in tomato plants. The outcomes of this research will provide critical insights into the development of a novel, sustainable tool for managing this major pest and the diseases transmitted by it, thereby contributing to the sustainability of Brazilian agriculture. (AU)