Osteogenic potential of bilayered membranes loaded with vitamin D3 and silver nanoparticles in in vitro and in vivo models.

• Grant number: 24/09160-5
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2025
• End date: March 31, 2027
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Manuela Rocha Bueno
• Grantee: Manuela Rocha Bueno
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil
• Associated researchers: Ana Luíza Garcia Millás Massaguer ; Claire Bardet ; Lucas Novaes Teixeira

Abstract

Vitamin D is a hormone with an endocrine mechanism of action that regulates calcium phosphate homeostasis and bone mineral metabolism. Despite its importance, vitamin D deficiency is a global public health problem, as approximately 1 billion people around the world are vitamin D deficient, while 50% of the population has vitamin D deficiency. Furthermore, studies suggest that the serum vitamin D concentration of individuals with periodontitis is lower than that of normal people, and scaling and root planing, together with vitamin D supplementation, have been shown to play a significant role in improving periodontal clinical parameters. Thus, the hypothesis is that PLGA bilayer membranes with antimicrobial and osteogenic properties loaded with silver nanoparticles and 1,25-dihydroxyvitamin D3 could influence the osteogenic and immunomodulatory potential of osteoblasts challenged or not with LPS from Porphyromonas gingivalis and in new bone formation in critical calvarial defect in rats. The present proposal aims to characterize bilayer membranes in their morphological and physicochemical characteristics and test them in primary culture osteoblasts with or without LPS from P. gingivalis, evaluating their effects on cell viability, expression of genes associated with bone metabolism, profile of cytokines and alkaline phosphatase activity. Furthermore, the aim is to evaluate the effects of the membrane on new bone formation in critical calvarial defects. Thus, it is expected to develop a safe and effective biomaterial to be used in the future in periodontal and/or peri-implant bone defects. (AU)