EVALUATION OF THE INFLUENCE OF PHYTOCANNABINOIDS THC, CBD AND CBG PRODUCED IN BRAZILIAN SOIL ON THE ACTIVITY OF DIFFERENT CELL LINES AND RELATED TO XENOGENOUS BIOMATERIAL: IN VITRO

• Grant number: 24/04185-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2025
• End date: July 31, 2027
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Michelle Bianchi de Moraes
• Grantee: Michelle Bianchi de Moraes
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

There is a growing interest in the use of cannabis for therapeutic purposes, mainly through phytocannabinoids, as a detailed discussion of the endocannabinoid system and its potential usefulness in pharmacotherapy is ongoing. Phytocannabinoids show their importance in relation to synthetic medications due to the "entourage" effect, that is, synergism between their substances, benefiting the results in treatment. The phytocannabinoids present in plants interact with the endocannabinoid system, which is responsible for a wide network of chemical signals and cellular receptors that are present throughout our brain and body. The most studied cannabinoids are ¿9-tetrahydrocannabinol (THC), cannabidiol (CBD), and more recently cannabigerol (CBG), however these studies do not include those planted in Brazilian soil, in order to support their use and clinical applicability, since the importance of soil and climate in the characteristics of the plant has already been proven. Therefore, this study aims to evaluate THC, CBD and CBG, produced in Brazilian soil, in different concentrations, in the activity of HFF1 fibroblast, HaCat keratinocyte and MC3T3-E1 osteoblast cell lines associated or not with xenogeneic biomaterial. The evaluations will be performed through the following tests: determination of cell viability, evaluation of morphology, cell migration and proliferation, total protein content and also cytokine tests, in the MC3T3-E1 osteoblast cell lines, in addition to the aforementioned tests, evaluations of alkaline phosphatase activity and formation of mineralization nodules will be included. The results will be statistically analyzed, initially through the normality test aiming at the application of the appropriate statistical test. The adopted significance level will be the conventional value of 5%. (AU)