Optimization of lentiviral transduction by lentiviral vectors

• Grant number: 25/13226-4
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2025
• End date: September 30, 2026
• Field of knowledge: Biological Sciences - Immunology
• Principal Investigator: Bruna Cunha Gondim de Alencar
• Grantee: Bruna Cunha Gondim de Alencar
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated research grant: 22/12443-3 - Role of myosin IXB in the regulation of inflammasome activation, AP.R
• Associated scholarship(s): 25/24990-7 - Optimization of lentiviral transduction by lentiviral vectors, BP.IC

Abstract

Although transduction of human macrophages by lentiviral vectors offers a powerful resource for gene knockdown, it faces several challenges that directly impact laboratory execution. The lack of methodological details in scientific studies leads to difficulties in reproducing existing protocols. The laboratory hosting the current project has encountered difficulties in genetically manipulating macrophages, a factor that prevents the use of this cell type in studies that require manipulations such as gene silencing. (AU)