Miniaturization of analytical instrumentation for separation techniques: sample preparation and printing of result

• Grant number: 05/59360-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: November 01, 2006
• End date: August 31, 2011
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Fernando Mauro Lanças
• Grantee: Fernando Mauro Lanças
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The commercial development of instrumental chromatography, which occurred from the decade of the 50s onwards, has enabled the analysis (qualitative and quantitative) of increasingly smaller analytes present in increasingly complex matrixes. However, the efforts begun particularly in the decade of the 80s, aimed at a miniaturization of these systems, and their innumerable advances, have still both reached the great user public. The large companies producing the equipment still continue to offer instruments very similar to those existing in the decade of the 80s, with small, usually inadequate adaptations. The experience of the proposing group in the last 15 years in the development of chromatographs and accessories (columns, detectors, data systems), extractors for supercritical fluids and accelerated extraction with solvents, micro-extraction in solid phase, and others, suggests that the best alternative for miniaturization of equipment for separation techniques, for the next 30 years, would be of intermediary dimensions and technology between the "macro” systems, popular at present, and the systems based on "microchips”, a probable alternative for a more distant future. With a real base in the experience involved with the development of various items of equipment and accessories for this area, what is proposed in this project is the conclusion of the miniaturization of a gas chromatograph under development; the development of a piece of equipment for micro-LC; the development of new components, interfaces and accessories for micro-LC, SPME and SBSE; and the development of new systems of acquisition and treatment of data for chromatography, as well as the application of the same in the analysis of various complex samples such as those of environmental interest (pollutants), public health (medicines in biological fluids, chemotherapies, and drugs of abuse), foodstuffs (pesticides and drinks) and fuels (with adulterants). (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA GOMES, PAULO C. F.; BARNES, BRIAN B.; SANTOS-NETO, ALVARO J.; LANCAS, FERNANDO M.; SNOW, NICHOLAS H.. Determination of steroids, caffeine and methylparaben in water using solid phase microextraction-comprehensive two dimensional gas chromatography-time of flight mass spectrometry. Journal of Chromatography A, v. 1299, p. 126-130, JUL 19 2013. (05/59360-0, 07/03844-4)

BARLETTA, JULIANA Y.; DE LIMA GOMES, PAULO C. F.; DOS SANTOS-NETO, ALVARO J.; LANCAS, FERNANDO M.. Development of a new stir bar sorptive extraction coating and its application for the determination of six pesticides in sugarcane juice. JOURNAL OF SEPARATION SCIENCE, v. 34, n. 11, p. 1317-1325, JUN 2011. (05/59360-0, 07/03844-4)

FEITOSA DE LIMA GOMES, PAULO CLAIRMONT; BARLETTA, JULIANA YAMASHITA; DOMINGUES NAZARIO, CARLOS EDUARDO; SANTOS-NETO, ALVARO JOSE; VON WOLFF, MARYA ANNE; REGANHAN CONEGLIAN, CASSIANA MARIA; UMBUZEIRO, GISELA ARAGAO; LANCAS, FERNANDO MAURO. Optimization of in situ derivatization SPME by experimental design for GC-MS multi-residue analysis of pharmaceutical drugs in wastewater. JOURNAL OF SEPARATION SCIENCE, v. 34, n. 4, p. 436-445, FEB 2011. (05/59360-0, 07/03844-4)

NAZARIO, CARLOS E. D.; LANCAS, FERNANDO M.. Determination of Diclofenac in Bovine Milk at Low Levels Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry. FOOD ANALYTICAL METHODS, v. 10, n. 7, p. 2490-2496, JUL 2017. (05/59360-0)

NAZARIO, CARLOS E. D.; SILVA, MEIRE R.; FRANCO, MARAISSA S.; LANCAS, FERNANDO M.. Evolution in miniaturized column liquid chromatography instrumentation and applications: An overview. Journal of Chromatography A, v. 1421, p. 18-37, NOV 20 2015. (12/22055-9, 14/07347-9, 05/59360-0)

MARINS COUTINHO, LINCOLN FIGUEIRA; DOMINGUES NAZARIO, CARLOS EDUARDO; MONTEIRO, ALESSANDRA MAFFEI; LANCSA, FERNANDO MAURO. Novel devices for solvent delivery and temperature programming designed for capillary liquid chromatography. JOURNAL OF SEPARATION SCIENCE, v. 37, n. 15, p. 1903-1910, AUG 2014. (05/59360-0)

DOMINGUES NAZARIO, CARLOS EDUARDO; DE LIMA GOMES, PAULO CLAIRMONT FEITOSA; MAURO LANCAS, FERNANDO. Analysis of fluoxetine and norfluoxetine in human plasma by HPLC-UV using a high purity C18 silica-based SPE sorbent. ANALYTICAL METHODS, v. 6, n. 12, p. 4181-4187, 2014. (05/59360-0, 07/03844-4)

BARREIRO, JULIANA CRISTINA; LUIZ, ANDERSON LUIGI; FERNANDES MACIEL, SUELLEN CRISTIANE; SOARES MACIEL, EDVALDO VASCONCELOS; LANCAS, FERNANDO MAURO. Recent approaches for on-line analysis of residues and contaminants in food matrices: A review. JOURNAL OF SEPARATION SCIENCE, v. 38, n. 10, p. 1721-1732, MAY 2015. (05/59360-0, 07/03844-4)