Mycobacteria of medical significance in Brazil: molecular characterization, interaction with the environment and with macrophages

• Grant number: 06/01533-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: December 01, 2006
• End date: March 31, 2010
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Sylvia Luisa Pincherle Cardoso Leão
• Grantee: Sylvia Luisa Pincherle Cardoso Leão
• Host Institution: Departamento de Microbiologia, Imunologia e Parasitologia. Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Celia Regina Whitaker Carneiro
• Associated researchers: Edna Freymüller Haapalainen ; Renato Arruda Mortara ; Rosa Maria Silva ; Terezinha de Jesus Andreoli Pinto
• Associated research grant(s): 09/11250-2 - Studies on biofilms and rapidly-growing mycobacteria, AV.EXT
• Associated scholarship(s): 08/07409-3 - Biofilm formation with Mycobacterium massiliense strains presenting smooth and rough phenotypes isolated from outbreaks and environmental sources, BP.MS; 08/01451-8 - Molecular caracterization of rapidly growing micobacteria associated or not to outbreaks in laparoscopic surgeries for the development of a quick identification test, BP.DR

Abstract

Non-tuberculous micobacteria (NTM) are emerging pathogens, with potential to cause infections in immunocompromized and also immunocompetent patients, frequently as a complication of invasive procedures, like ophthalmologic, plastic, and laparoscopic surgeries, cosmetic procedures, and endoscopically based exams. Studies that improve our knowledge about the biology of these bacteria and the interaction with the environment and host cells will be useful for future elaboration of control programs and therapeutic approaches. In sub-project 1, three molecular methods will be used to type isolates from an outbreak of infections after surgical procedures that occurred at Belém (PA) between 2004 and 2005. The results will be compared with patients' data for analysis of epidemiological correlation. A bank of genetic profiles will be organized and a fluxogram will be elaborated to help in the management of future outbreaks. In sub-project 2, we will investigate the presence of plasmids and mycobacteriophages in outbreak and environment isolates and their role in horizontal transfer of genetic information. In sub-project 3, the potential of these bacteria to produce and to survive in biofilms will be evaluated and the role of these structures in resistance to biocides commonly used for equipment disinfection will be investigated. In sub-project 4, the interaction of a M. avium virulent isolate with epithelioid-like cells (recombinant interleucine-4 treated murine peritoneal macrophages), constituting an in vitro model developed by our group, will be investigated to improve our understanding of the functions of epithelioid cells present in granuloma. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA-NIERO, CRISTINA; LIMA, KARLA VALÉRIA BATISTA; LOPES, MARIA LUIZA; RABELLO, MICHELLE CHRISTIANE DA SILVA; MARSOLA, LOURIVAL RODRIGUES; BRILHANTE, VÂNIA CRISTINA RIBEIRO; DURHAM, ALAN MITCHEL; LEÃO, SYLVIA CARDOSO. Molecular characterization of Mycobacterium massiliense and Mycobacterium bolletii in isolates collected from outbreaks of infections after laparoscopic surgeries and cosmetic procedures. Journal of Clinical Microbiology, v. 46, n. 3, p. 850-855, Mar. 2008. (06/01533-9)

DA SILVA RABELLO, MICHELLE CHRISTIANE; MATSUMOTO, CRISTIANNE KAYOKO; PAULA DE ALMEIDA, LUIZ GONZAGA; CARMEN MENENDEZ, MARIA; DE OLIVEIRA, ROSANGELA SIQUEIRA; SILVA, ROSA MARIA; JESUS GARCIA, MARIA; LEAO, SYLVIA CARDOSO. First Description of Natural and Experimental Conjugation between Mycobacteria Mediated by a Linear Plasmid. PLoS One, v. 7, n. 1, JAN 3 2012. (06/01533-9)

L'ABBATE, CAROLINA; CIPRIANO, IVONE; PEREZ-HURTADO, ELIZABETH CRISTINA; LEAO, SYLVIA CARDOSO; WHITAKER CARNEIRO, CELIA REGINA; MACHADO, JR., JOEL. TGF-beta-Mediated Sustained ERK1/2 Activity Promotes the Inhibition of Intracellular Growth of Mycobacterium avium in Epithelioid Cells Surrogates. PLoS One, v. 6, n. 6, JUN 22 2011. (06/01533-9)

LEAO, SYLVIA CARDOSO; MATSUMOTO, CRISTIANNE KAYOKO; CARNEIRO, ADRIANA; RAMOS, ROMMEL THIAGO; NOGUEIRA, CHRISTIANE LOURENCO; LIMA JUNIOR, JAMES DALTRO; LIMA, KARLA VALERIA; LOPES, MARIA LUIZA; SCHNEIDER, HORACIO; AZEVEDO, VASCO ARISTON; et al. The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1 beta Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp bolletii. PLoS One, v. 8, n. 4, APR 2 2013. (06/01533-9)

RABELLO, MICHELLE CHRISTIANE DA SILVA; DE OLIVEIRA, ROSANGELA SIQUEIRA; SILVA, ROSA MARIA; LEAO, SYLVIA CARDOSO. Natural Occurrence of Horizontal Transfer of Mycobacterium avium-Specific Insertion Sequence IS1245 to Mycobacterium kansasii. Journal of Clinical Microbiology, v. 48, n. 6, p. 2257-2259, JUN 2010. (06/01533-9)

MATSUMOTO, CRISTIANNE KAYOKO; CHIMARA, ERICA; BOMBARDA, SIDNEY; DUARTE, RAFAEL SILVA; LEAO, SYLVIA CARDOSO. Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates. Journal of Clinical Microbiology, v. 49, n. 1, p. 62-68, JAN 2011. (08/01451-8, 06/01533-9)