Gene expression apoptosis after focal brain ischemia in rats and neutoprotective effect of hypotermia and ketoprofen

• Grant number: 05/00381-8
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2006
• End date: May 31, 2009
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Benedicto Oscar Colli
• Grantee: Benedicto Oscar Colli
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Brain ischemia is one of the most relevant of the human diseases, with high mortality and morbidity rates worldwide. It is a frequent condition in clinical practice that has no simple therapeutical solution, mainly due to the difficulties in performing a physiopathological study, which arise from the great diversity in its anatomical localization, its etiology and clinical manifestations. The aim of this work is to evaluate the expression pattern of genes related to apoptosis (NMDA, FAS, c-FLIP, caspase-8, caspase-3 and HSP70), either alone or associated to the neuroprotective effect of hypothermia and/or ketoprofen. To achieve this goal we will use an experimental model in which rats will be induced to develop focal brain ischemia by the occlusion of the midlle cerebral artery with an intraluminal probe and the results will be assessed by semi-quantitative polymerase chain reaction (RT-PCR) and real-time quantitative polymerase chain reaction (RQ-PCR). Wistar adult rats will be divided in three experimental groups: 1) control group, which comprises animals that will receive anesthesia only; 2) sham group, which comprises animals that will undergo a mock surgical procedure; 3) ischemic group, which will be further divided into four subgroups: Ischemic (SgI), Ischemic with hypothermia (SgHI), ischemic with ketoprofen (SgIC), and ischemic with hypothermia and ketoprofen (SgIHC). All animals within the ischemic subgroups will be induced to develop brain ischemia for one hour, sacrificed, and samples will be collected and further analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANIELA P. C TIRAPELLI; MUCIO LUIZ DE ASSIS CIRINO; CAMILA A. MELO DE CARVALHO; PAULO CEZAR NOVAIS; RAFAEL ZIMAK FIGUEREDO; LUCAS BARBOSA PORSANI; ISABELLA STRACIERI GULA; ANDRESSA ROMUALDO RODRIGUES; JAIRO PINHEIRO DA SILVA; ADRIANA LIS PEREIRA; et al. Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen. INTERNATIONAL JOURNAL OF MORPHOLOGY, v. 38, n. 3, p. 523-529, 2020-06-00. (05/00381-8)

DA CUNHA TIRAPELLI, DANIELA PRETTI; CARLOTTI JUNIOR, CARLOS GILBERTO; LEITE, JOAO PEREIRA; TIRAPELLI, LUIS FERNANDO; COLLI, BENEDICTO OSCAR. Expressão de HSP70 na isquemia cerebral e a ação neuroprotetora da hipotermia e do cetoprofeno. Arquivos de Neuro-Psiquiatria, v. 68, n. 4, p. 592-596, AUG 2010. (05/00381-8)

TIRAPELLI, D. P. C.; CARLOTTI JR, C. G.; LEITE, J. P.; LIZARTE, F. S. N.; TIRAPELLI, L. F.; COLLI, B. O.. Analysis of the NMDA in Focal Cerebral Ischemia in Rats. INTERNATIONAL JOURNAL OF MORPHOLOGY, v. 30, n. 3, p. 979-985, 2012. (05/00381-8)