Evaluation between PTEN, Akt, Mdm2 and p53 signaling pathways in different HNSCC cell lines treated with epidermal growth factor and 17-AAG

• Grant number: 06/06728-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2007
• End date: January 31, 2009
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Decio dos Santos Pinto Junior
• Grantee: Decio dos Santos Pinto Junior
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Head and neck squamous cell carcinoma (HNSCC) represents 90% of all head and neck malignancies. Cancer growth, invasion and metastasis are due to several signaling pathways that, unfortunately, are not completely understood. The aim of this study will be the crosstalk evaluation between PTEN, Akt, Mdm2 and p53 signaling pathways in four different HNSCC cell lines (HN6, HN19, HN30 and HN31) and HaCat cell line (immortalized keratinocytes), treated with 10ng/ml EGF (epidermal growth factor) and 17-AAG. Western blot and imunofluorescence will be perform in order to analyze PTEN and PI3K signaling key target proteins: PTEN, Akt, Mdm2 and p53. (AU)