Role of innate immunity receptors beta-glucan receptor, MR, TLR2 and TLR4 for recocgnition of Candida albicans by human monocytes stimulated with extracts of Agaricus braziliensis

• Grant number: 06/06925-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2007
• End date: February 28, 2009
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Ramon Kaneno
• Grantee: Ramon Kaneno
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The phagocytic function can be increased through the cell activation for a variety of surface molecules, among them we can detach the pattern-recognition receptors (PRR) such as Toll like receptors (TLRs), the mannose receptors (MR) and the beta-glucan receptors. Considering the importance that such receptors for recognition of pathogens and activation of phagocytic cells, it is possible to think that agents able capable to stimulate their expression of surface receptors can be potentially useful as adjuvant in the treatment of infections by C. albicans. Supporting this hypothesis, we have previously observed that peritoneal macrophages of mice treated with a peptidoglycans-rich extract obtained of the mushroom A. brasiliensis (acid-treated ammonium oxalate-soluble fraction - ATF), show higher candidacidal capacity than normal animals inoculated with PBS. It was also associated with higher production of H2O2 and expression of MR. The present project was designed to test the hypothesis that polysaccharides of the A. brasiliensis increase the activity of human monocytes by modulating the expression of surface receptors related to innate immunity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, PRISCILA RAQUEL; VICTORIANO DE CAMPOS SOARES, ANGELA MARIA; DA SILVA PINTO DOMENEGHINI, ANDREA VANESSA; GOLIM, MARJORIE ASSIS; KANENO, RAMON. Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, MAR 23 2017. (06/06925-2)