Evaluation of the surgical thecnique, post operative systemic inflamatory response, stress and pain in bitches following transvaginal laparoscopic (notes), video-assisted and conventional ovariohisterectomy

• Grant number: 09/14712-7
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2009
• End date: November 30, 2011
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Gilson Hélio Toniollo
• Grantee: Gilson Hélio Toniollo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The contraceptive surgical procedures aim to inhibit the oestrus, to avoid or treat diseases of the reproductive tract and to control the population of companion animals. However, such surgical procedures are painful and result in post operative pain and severe inflammation. Therefore, the development of new minimally invasive techniques is required. The present study aims to compare the systemic inflammatory response, post operative pain and stress and to evaluate the surgical techniques of ovariohysterectomy, performed by transvaginal laparoscopy, transabdominal signle-port technique or conventionally in bitches. Thirty female canines will be selected and divided into one of three groups: transvaginal laparoscopic ovariohysterectomy (TVL-OHE, group I); single-port laparoscopic-assisted ovariohysterectomy (SPLA-OHE, group II); or median celiotomic ovariohysterectomy with the use of the spay Snook hook (OHE, group III). The assessment of the surgical techniques will involve the mean operational cost and the mean time of surgical procedure in all groups. Post operative pain and stress will be determined by means of visual analogue pain scale evaluation and serum cortisol and glucose dosage in the moments M0, M1, M2, M3 M4 and M5, which represent the pre operative period and 1, 3, 6, 12 and 24 hours following orotraqueal extubation. The evaluation of the inflammatory response will be obtained by means of serum dosage of IL-1, IL-6 and TNF-±, PCR and SAA, in the moments T0, T1, T2, T3 T4 and T5, which represent the firts hour before the surgical procedure and 4, 8, 24, 72, 144 hours of post operative period. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, M. A. M.; TONIOLLO, G. H.; FLORES, F. N.; VALADAO, C. A. A.; MEDEIROS, R. M.; CARDILLI, D. J.; GERING, A. P.; TEIXEIRA, P. P. M.; SERAFINI, G. M. C.; COUTINHO-JUNIOR, A. S.; et al. Tempo cirúrgico e das complicações entre as técnicas de ovário-histerectomia totalmente transvaginal (total-NOTES), videoassistida com único portal e convencional em cadelas. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 67, n. 3, p. 647-654, JUN 2015. (09/14712-7)