Evaluation of oxidative DNA damage in lymphocytes, oxidative stress and cytokines profile in plasma from patients submitted to surgery and inhalational anesthesia with sevoflurane

• Grant number: 09/51110-5
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2009
• End date: August 31, 2011
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Jose Reinaldo Cerqueira Braz
• Grantee: Jose Reinaldo Cerqueira Braz
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Some anesthetics have been shown as being mutagenic for men. However, little is known about the genotoxic potential of sevoflurane (SVF), one of the most used agents in anesthesia. Patients have stress when submitted to anesthesia and surgery, characterized by inflammatory response, and oxidative stress because of the trauma of the surgery, and possibly due to the anesthetics. The study aims to evaluate the genotoxicity of the halogenated anesthetic SVF in lymphocytes, the cytokines profile (IL-1, IL-6, IL-8, IL-10, IL-12, TNF-α), and oxidative stress in plasma from patients submitted to surgery. DNA damage will be performed by the comet assay, the cytokines by flow cytometry and oxidative stress by total antioxidant performance (TAP). The study will be conducted on 30 individuals of both genders, ASA I status, between 18 and 50 years. Two groups of 15 individuals will be evaluated: SVF group, patients undergoing surgery during, at least, 120 minutes under 1.0 to 1.5 minimum alveolar concentration (MAC) of SVF (2% to 3%). Blood samples will be collected before anesthesia (M1), after anesthesia induction and before the beginning of the surgery (M2), 120 min after anesthesia induction (M3) and and on the postoperative first day (M4). The control group (C) will be consisted of 15 volunteers and blood sample will be collected a single time. It is expected that the results may contribute to the establishment of strategies aimed at reducing potential risks to individuals exposed to SVF and for the better understanding of its effects on the DNA, cytokines and oxidative stress. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRAZ, MARIANA G.; BRAZ, LEANDRO G.; BARBOSA, BARBARA S.; GIACOBINO, JULIANA; OROSZ, JOSE E. B.; SALVADORI, DAISY M. F.; BRAZ, JOSE R. C.. DNA damage in patients who underwent minimally invasive surgery under inhalation or intravenous anesthesia. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 726, n. 2, p. 251-254, DEC 24 2011. (09/51110-5)

OROSZ, JOSE EDUARDO B.; BRAZ, LEANDRO G.; FERREIRA, ANA LUCIA A.; AMORIM, ROSA BEATRIZ; SALVADORI, DAISY MARIA F.; YEUM, KYUNG-JIN; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Balanced anesthesia with sevoflurane does not alter redox status in patients undergoing surgical procedures. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 773, p. 29-33, OCT 2014. (09/51110-5)

SILVA, MARIANE A. P.; BRAZ, LEANDRO G.; BRAZ, JOSE REINALDO C.; BRAZ, MARIANA G.. Modulation of gene expression and inflammation but not DNA damage after sevoflurane anesthesia. Environmental and Molecular Mutagenesis, v. 64, n. 5, p. 6-pg., 2023-04-13. (09/51110-5)

OROSZ, JOSE EDUARDO B.; BRAZ, MARIANA G.; GOLIM, MARJORIE A.; BARREIRA, MARCIO ANTONIO A.; FECCHIO, DENISE; BRAZ, LEANDRO G.; BRAZ, JOSE REINALDO C.. Cytokine Profile in Patients Undergoing Minimally Invasive Surgery with Balanced Anesthesia. Inflammation, v. 35, n. 6, p. 1807-1813, DEC 2012. (09/51110-5)