In vitro expression of cyclooxygenase-2 (COX2) for osteosarcoma exposed to a COX2 selective inhibitor

• Grant number: 09/53493-9
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2010
• End date: December 31, 2011
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Noeme Sousa Rocha
• Grantee: Noeme Sousa Rocha
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• City of the host institution: Botucatu

Abstract

Osteosarcoma (OSA) is a neoplasia that affects the bone of man and other animals. In Brazil the canine OSA is poorly studied, even though it represents 80% of the bone tumors in dogs and since it holds identical biology of the human OSA it might be a good study model. However, primary bone neoplasia is not so frequent when compared to tumors from other organs. Nevertheless, OSA is important due to the difficulties found in dealing with it, since it presents a good range of lesions, some are innocuous, and some are rapidly lethal. This work aims to develop and characterize six spontaneous canine osteosarcoma primary cultures and then they will be divided into three cyclooxygenase-2 (Cox2) positive cultures and three Cox2 negative ones. For that, it is necessary to investigate the in vitro expression of Cox2 in these different cultures of primary osteosarcoma previously exposed to Cox2 selective inhibitor. The cells will then be cultured in DMEM high glucose medium and identified by alkaline phosphatase, calcium ion, osteocalcin, osteopontin and osterix biomarkers. Later, a panel of these cultures will be created and they will be exposed to other biomarkers such as: vimentin, cytokeratin, caspase-3, p53, Bcl-2, Bax, VEGF, MMP-2, MMP-9 and Ki-67. The results of this study may help cancer researches and may bring new knowledge of the carcinogenesis of this tumor, so it can support new therapeutical approaches and thus to improve the quality of life for patients with cancer. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAGNINI, DIDIER Q.; SALGADO, BRENO S.; LINARDI, JULIANA L.; GRANDI, FABRIZIO; ROCHA, RAFAEL M.; ROCHA, NOEME S.; TEIXEIRA, CARLOS R.; DEL PIERO, FABIO; SEQUEIRA, JULIO L.. Ocular melanoma and mammary mucinous carcinoma in an African lion. BMC Veterinary Research, v. 8, SEP 25 2012. (10/51596-2, 09/53493-9, 11/01610-1)

CINEGAGLIA, NAIARA COSTA; OLIVEIRA BERSANO, PAULO RICARDO; ABIGAIL MENDES ARAUJO, MARIA JOSE; BUFALO, MICHELLE CRISTIANE; SFORCIN, JOSE MAURICIO. Anticancer Effects of Geopropolis Produced by Stingless Bees on Canine Osteosarcoma Cells In Vitro. Evidence-based Complementary and Alternative Medicine, v. 2013, p. 6-pg., 2013-01-01. (09/53493-9)

CINEGAGLIA, NAIARA COSTA; OLIVEIRA BERSANO, PAULO RICARDO; ABIGAIL MENDES ARAUJO, MARIA JOSE; BUFALO, MICHELLE CRISTIANE; SFORCIN, JOSE MAURICIO. Anticancer Effects of Geopropolis Produced by Stingless Bees on Canine Osteosarcoma Cells In Vitro. Evidence-based Complementary and Alternative Medicine, 2013. (09/53493-9)

BERNARDINO, PEDRO NEGRI; OLIVEIRA BERSANO, PAULO RICARDO; LIMA NETO, JOAO FERREIRA; SFORCIN, JOSE MAURICIO. Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells. BIOMEDICINE & PHARMACOTHERAPY, v. 104, p. 268-274, AUG 2018. (09/53493-9, 12/21158-9)

CINEGAGLIA, N. C.; BERSANO, P. R. O.; BUFALO, M. C.; SFORCIN, J. M.. Cytotoxic Action of Brazilian Propolis In Vitro on Canine Osteosarcoma Cells. Phytotherapy Research, v. 27, n. 9, p. 1277-1281, SEP 2013. (09/53493-9)