Action mechanisms and expression analysis of cyclin D1, akt, mTOR and hsp90 proteins in squamous cell carcinoma in vitro after low intensity laser irradiation

• Grant number: 09/16183-1
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2010
• End date: December 31, 2012
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Suzana Cantanhede Orsini Machado de Sousa
• Grantee: Suzana Cantanhede Orsini Machado de Sousa
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aim of the present study is to evaluate expressions and action mechanism of the proteins: AKT, Cyclin D1, mTOR and Hsp90, in oral squamous cell carcinoma, normal keratinocyte and dysplastic keratinocyte cell lines after low power laser irradiation. Two light wavelenghts will be used: 660 and 780nm, and three different dosages: 2.05; 3.07; 6.15 J/cm2. After irradiation the cells will be counted, and a qualitative and quantitative analysis of proteins amount will be performed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SPERANDIO, FELIPE F.; GIUDICE, FERNANDA S.; CORREA, LUCIANA; PINTO, DECIO S., JR.; HAMBLIN, MICHAEL R.; DE SOUSA, SUZANA C. O. M.. Low-level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway. Journal of Biophotonics, v. 6, n. 10, p. 9-pg., 2013-10-01. (09/08452-2, 09/16183-1)

SPERANDIO, FELIPE F.; GIUDICE, FERNANDA S.; CORREA, LUCIANA; PINTO, JR., DECIO S.; HAMBLIN, MICHAEL R.; DE SOUSA, SUZANA C. O. M.. Low-level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway. Journal of Biophotonics, v. 6, n. 10, SI, p. 839-847, OCT 2013. (09/16183-1, 09/08452-2)