Biomechanical and histological effects of coating a polypropylene mesh with a purified collagen gel: experimental study

• Grant number: 10/01757-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2010
• End date: June 30, 2012
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Cássio Luís Zanettini Riccetto
• Grantee: Cássio Luís Zanettini Riccetto
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Benedicto de Campos Vidal ; Rodrigo Teixeira Siniscalchi

Abstract

The most used synthetic material (mesh) for the treatment to of vaginal walls prolapses of nowadays is the monofilament polypropylene, with rates of healing up to 90%. It is known, however, that the complications related to the integration are more prevalent. For being a biologically compatible material, little immunogenic and with modulating properties of the inflammatory process, the collagen can be used as an important healing agent improving the integration of the meshes.Aim: To study the biomechanical and histological effects of recovering the monofilament polypropylene meshes with a high purified collagen gel. Material and Method: Fifteen rats will be used for the histological study and another 15 for the biomechanical study. On one side of the animal's abdominal wall, a monofilament polypropylene mesh will be placed and on the other side, a similar mesh recovered with purified collagen gel. The animals of each group will be divided into three sub-groups each with 5 animals to be euthanized at 7 and 30 and 90 days after implantation. In the histological study the characteristics of the intensity of the inflammatory and fibroblastic reaction will be analyzed. The density of the collagen fibers will be studied through the stereological method and through polarization microscopy. The biomechanical analysis will be conducted in an accurate tensiometer in which the objective is to quantify the adhesion of meshes to the surrounding tissues. (AU)

Filed patent(s) as a result of this research project