Regulation of dentin-pulp and periodontal ligament cells in healthy individuals and with hypophosphatasia

• Grant number: 08/00534-7
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2008
• End date: December 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Márcio Zaffalon Casati
• Grantee: Márcio Zaffalon Casati
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The aims of this study will be: (1) To determine basal differences in gene expression in pulp vs. periodontal ligament (PDL) cells in healthy and hypophosphatasia-diagnosed (HPP) subjects, focusing on genes associated with differentiation/mineralization and inorganic phosphate (Pi) metabolism; (2) To determine the effect that Pi treatment exerts on pulp vs. PDL cells, in vitro. To obtain primary cells cultures of the pulp and PDL, patients between 16 and 20 years old that have third molar submerged extracted for orthodontic reasons will be identified and classified at two groups: A - control group (healthy subjects), B - test group (hypophosphatasia-diagnosed subjects). Proliferation/viability assay and mineralization in vitro, and evaluation of mRNA with a quantitative real-time PCR will be performed. Mean and standard deviation (S.D.) will be calculated to establish statistical comparisons at a level of significance of 5%. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, THAISANGELA L.; FOSTER, BRIAN L.; SILVERIO, KARINA G.; MARTINS, LUCIANE; CASATI, MARCIO Z.; SALLUM, ENILSON A.; SOMERMAN, MARTHA J.; NOCITI, JR., FRANCISCO H.. Correction of Hypophosphatasia-Associated Mineralization Deficiencies In Vitro by Phosphate/Pyrophosphate Modulation in Periodontal Ligament Cells. Journal of Periodontology, v. 83, n. 5, p. 653-663, MAY 2012. (08/00534-7, 07/08192-5)

RODRIGUES, THAISANGELA L.; NAGATOMO, KANAKO J.; FOSTER, BRIAN L.; NOCITI, JR., FRANCISCO H.; SOMERMAN, MARTHA J.. Modulation of Phosphate/Pyrophosphate Metabolism to Regenerate the Periodontium: A Novel In Vivo Approach. Journal of Periodontology, v. 82, n. 12, p. 1757-1766, DEC 2011. (08/00534-7)

MARTINS, LUCIANE; RODRIGUES, THAISANGELA L.; RIBEIRO, MARIANA MARTINS; SAITO, MIKI TAKETOMI; OLIVEIRA GIORGETTI, ANA PAULA; CASATI, MARCIO Z.; SALLUM, ENILSON A.; FOSTER, BRIAN L.; SOMERMAN, MARTHA J.; NOCITI, JR., FRANCISCO H.. Novel ALPL genetic alteration associated with an odontohypophosphatasia phenotype. BONE, v. 56, n. 2, p. 390-397, OCT 2013. (08/00534-7, 07/08192-5)

RODRIGUES, THAISANGELA L.; FOSTER, BRIAN L.; SILVERIO, KARINA G.; MARTINS, LUCIANE; CASATI, MARCIO Z.; SALLUM, ENILSON A.; SOMERMAN, MARTHA J.; NOCITI, JR., FRANCISCO H.. Hypophosphatasia-associated Deficiencies in Mineralization and Gene Expression in Cultured Dental Pulp Cells Obtained from Human Teeth. JOURNAL OF ENDODONTICS, v. 38, n. 7, p. 907-912, JUL 2012. (08/00534-7, 07/08192-5)