The polymorphism - 251 A/T on the promoter region of the interleukin 8 gene and the risk of gastric cancer in Helicobacter pylori infected patients: case-control study

• Grant number: 08/05763-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2008
• End date: July 31, 2010
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Nora Manoukian Forones
• Grantee: Nora Manoukian Forones
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The Gastric Cancer (GC) shows a high incidence globally with about 700,000 died patients per year. In Brazil, during 2006, it was observed 23,200 new GC cases between men and women; in the United States the estimative of new cases of GC for 2007 was of 21,260 resulting in death of 11,210 people. The bacteria Helicobacter pylori is one of the main factors of risk for inside unchaining diverse inflammatory answers from the host's gastric epithelium. Example of this is the induction of the transcription of the factor of activation of protein 1 (AP-1), activating the nuclear factor (NF)-ºB and finally the release of Interleukin 8 (IL-8), that it attracts phagocytes being able to cause damages to the gastric mucosa for the release of radicals of reactive oxygen, beyond inducing the cellular proliferation and angiogenesis. Some authors describe the involvement of the polymorphism -251A/T in the promotional region of the gene of the IL-8 with the incidence, prevalence and/or prognostic in other illnesses as in the tuberculosis, neonates respiratory stress syndrome, colorectal cancer, bronchial asthma, viral respiratory infections and prostatic cancer. This project aims at to carry through comparative analyses of genotypes AA, AT and TT in the promoter region of the gene of the IL-8 (-251A/T), enters the group in case that 1 (patient with GC), group case 2 (relatives of 1st degree) and of the group it has controlled. To suggest which allele of this promoter region of the IL-8 increases the risk in developing GC. To correlate the presence or not of this polymorphism in the patients with GC with its respective relatives of 1st degree and to the group has not controlled. To correlate the polymorphism -251A/T in the promoter region of the gene of the IL-8 with the infection for H. pylori. The DNA will be extracted of leukocytes, purified and the analysis of the polymorphism -251A/T in the promoter region of the gene of the IL-8 will be genotyped by the PCR-RFLP technique and the results will be visualized through 5% agarose gel electrophoresis with ethide bromide. (AU)