Analise de expressao e mutagenese de mecanismo de patogenicidade de xanthomonas axonopodis pv. citri: o sistema de secrecao tipo ii.

• Grant number: 08/00070-0
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2009
• End date: February 28, 2010
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Alexandre Morais Do Amaral
• Grantee: Alexandre Morais Do Amaral
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil
• City of the host institution: Campinas

Abstract

The citrus canker is, certainly, one of the most destructive diseases of plants worldwide, especially in those countries where the citrus industry plays a strategic role that results in job and incomes, which includes Brazil, the greatest orange producer. Therefore, Brazil sponsored the sequencing of the causal agent of citrus canker, the bacterium Xanthomonas axonopodis pv. citri (Xac). The objective of this work was to genetically identify the biological tools that the bacterium harbors and that utilizes during the infection of citrus plants. Thus, the prospects are to better understand how the bacterium acts and, therefore, identify targets for the management of the disease. During its complete sequencing, various secretory systems were identified, which are highly related to pathogenesis. These systems include the "type two secretory system" (T2SS), which in other plant bacterial pathogens is involved in the process of infection. Strikingly, in Xac, there are two distinct copies of the T2SS - which is not frequent in other bacteria. However, to our knowledge, there is no study that investigated the role played by these systems in the occurrence of citrus canker. The goal of thei study is to characterize the expression of the two copies of the T2SS in Xac during the infection. To perform the characterization, the expression will be monitored by RT-qPCR along with the technique of mutagenesis. Finally, the aim is to identify the correlation - if there is any - between the two T2SS and how they contribute to the citrus canker to succeed. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

BAPTISTA, JULIANA CRISTINA; MACHADO, MARCOS ANTONIO; HOMEM, RAFAEL AUGUSTO; TORRES, PABLO SEBASTIAN; VOJNOV, ADRIAN ALBERTO; DO AMARAL, ALEXANDRE MORAIS. Mutation in the xpsD gene of Xanthomonas axonopodis pv. citri affects cellulose degradation and virulence. GENETICS AND MOLECULAR BIOLOGY, v. 33, n. 1, p. 146-153, 2010. (08/00070-0)