| Grant number: | 10/10949-0 |
| Support Opportunities: | Regular Research Grants |
| Start date: | October 01, 2010 |
| End date: | November 30, 2011 |
| Field of knowledge: | Health Sciences - Medicine - Medical Clinics |
| Principal Investigator: | Sabri Saeed Mohammed Ahmed Al-Sanabani |
| Grantee: | Sabri Saeed Mohammed Ahmed Al-Sanabani |
| Host Institution: | Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
| Associated researchers: | Ester Cerdeira Sabino ; Israel Bendit |
Abstract
Aims: We aimed to investigate the prevalence, characterization and possible disease association of erythrovirus genotypes variants in a acute lymphoblastic and myeloblastic leukemia patients group using the molecular approaches for accessing partial and complete genome sequences of this virus.Methods: Three hundred twenty bone marrow (BM) samples from patients with acute lymphoblastic (n=69) and myeloblastic (n=251) leukemia at diagnosis, followed in our institute since march 2008, will be analysed for erythrovirus genotypes DNA by previous published methods of nested polymerase chain reaction (nested PCR) for partial erythrovirus NS1 region and near full length genome. Direct DNA sequencing of nested PCR products from the NS1 region and near full length genome will be performed to differentiate and determine the subtypes. Appropriate phylogenetic and statistical methods will be used for subsequents epidemiological, structural and possible disease association studies. (AU)
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