Characterization site specific of the oral microflora in the ideal study group

• Grant number: 10/51716-8
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2010
• End date: October 31, 2012
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Francisco Gorgônio Nóbrega
• Grantee: Francisco Gorgônio Nóbrega
• Host Institution: Faculdade de Odontologia (FOSJC). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The oral cavity is the first segment of the digestive tract and is composed of many different structures that makes it a complex and diverse ecosystem for microbial growth. For a long time the traditional microbiology focused his studies on isolation and identification of species. However, it has become clear that the state of health or disease depends on the relationship between host and microbial community as a whole: Therefore, understanding the oral microbiota in health seems to be essential for understanding the disease process. This study proposes to characterize the microbial populations that colonize different oral sites in systemic healthy individuals with a pattern of optimal oral health. Participants will six young adults (ages 18 to 25 years) of both genders, healthy and with a pattern of optimal oral health, backed up by thorough medical history and clinical standards. Saliva samples will be collected, supra and subgingival biofilm and several oral microenvironments, such as the dorsum of the tongue, buccal mucosa, hard palate, soft palate, gingiva, the floor of mouth, anterior vestibule, and tonsils. For a highly representative of the genetic material of each microbial community collected will be used the technique of pyrosequencing. From the characterization of the ideal profile of this group, we intend to lay a solid foundation for future studies wishing to evaluate changes that occur in the microbiota in different conditions or disease state. (AU)